4a (NC_002645) Virus Tagged ORF Clone

CAT#: VC100564

Myc-DDK-tagged ORF clone of viral ORF for 4a protein [Human coronavirus 229E], codon optimized for human cell expression, NP_073552

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 4a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100564 represents NCBI reference of NP_073552 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGGGTCTGTTCACACTCCAGCTCGTCAGTGCGGTGAATCAGTCCCTTTCCAACGCCAAGGTGT
  CTGCTGAGGTGTCACGCCAGGTTATACAGGACGTCAAGGACGGTACTGTCACCTTCAACCTTCTCGCTTA
  CACTCTGATGTCCCTTTTTGTCGTCTATTTTGCATTGTTCAAAGCCAGGTCCCACCGCGGTCGGGCCGCC
  CTGATCGTGTTCAAAATTCTGATTCTGTTCGTTTACGTGCCTCTGCTGTACTGGTCTCAGGCATATATCT
  ACGCAACATTGATTGCCGTGATATTGCTCGGTAGGTTTTTTCATACCGCATGGCACTGCTGGCTTTACAA
  AACCTGGGATTTCATCGTGTTCAACGTGACTACGCTCTGCTACGCCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100564 representing NP_073552
  Red=Cloning sites Green=Tags
  
  MALGLFTLQLVSAVNQSLSNAKVSAEVSRQVIQDVKDGTVTFNLLAYTLMSLFVVYFALFKARSHRGRAA
  LIVFKILILFVYVPLLYWSQAYIYATLIAVILLGRFFHTAWHCWLYKTWDFIVFNVTTLCYAR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_002645
• ORF Size: 399 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_002645.1, NP_073552
• RefSeq ORF: 399
• Locus ID: 918759
• MW: 15.3 kDa