M (NC_002645) Virus Tagged ORF Clone

CAT#: VC100567

Myc-DDK-tagged ORF clone of viral ORF for membrane protein [Human coronavirus 229E], codon optimized for human cell expression, NP_073555

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: M
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100567 represents NCBI reference of NP_073555 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAATGACAACTGCACTGGCGATATTGTGACACACCTGAAAAATTGGAATTTTGGCTGGAACGTGA
  TTCTCACGATTTTTATAGTAATCCTCCAGTTCGGACATTATAAGTACTCAAGACTTTTCTATGGACTGAA
  GATGCTCGTTCTCTGGTTGCTGTGGCCATTGGTTCTGGCGCTCAGCATCTTCGACACATGGGCGAATTGG
  GATTCAAACTGGGCATTTGTTGCATTCTCTTTTTTCATGGCAGTTTCTACCCTGGTCATGTGGGTCATGT
  ATTTCGCAAATAGCTTCAGGCTGTTCAGGCGCGCTCGGACGTTTTGGGCTTGGAACCCTGAAGTCAACGC
  CATAACCGTCACCACAGTTCTCGGGCAAACGTATTACCAGCCTATTCAACAGGCCCCTACTGGCATTACC
  GTTACCCTGCTGTCAGGTGTGTTGTACGTTGATGGCCATAGGCTGGCTAGCGGGGTGCAGGTTCATAACC
  TGCCTGAATACATGACCGTGGCCGTGCCGAGTACAACTATCATTTATAGTCGAGTGGGGAGAAGCGTTAA
  CTCACAGAATAGTACCGGCTGGGTGTTCTATGTGCGGGTGAAGCACGGCGATTTCTCTGCGGTCAGCAGC
  CCCATGTCTAATATGACCGAAAACGAGCGGCTGCTGCACTTTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100567 representing NP_073555
  Red=Cloning sites Green=Tags
  
  MSNDNCTGDIVTHLKNWNFGWNVILTIFIVILQFGHYKYSRLFYGLKMLVLWLLWPLVLALSIFDTWANW
  DSNWAFVAFSFFMAVSTLVMWVMYFANSFRLFRRARTFWAWNPEVNAITVTTVLGQTYYQPIQQAPTGIT
  VTLLSGVLYVDGHRLASGVQVHNLPEYMTVAVPSTTIIYSRVGRSVNSQNSTGWVFYVRVKHGDFSAVSS
  PMSNMTENERLLHFF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_002645
• ORF Size: 675 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_002645.1, NP_073555
• RefSeq ORF: 675
• Locus ID: 918762
• MW: 26.0 kDa