E (NC_002645) Virus Tagged ORF Clone

CAT#: VC100566

Myc-DDK-tagged ORF clone of viral ORF for envelope protein [Human coronavirus 229E], codon optimized for human cell expression, NP_073554

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100566 represents NCBI reference of NP_073554 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCTCAAGCTTGTTGACGACCATGCACTGGTGGTAAACGTGCTTCTCTGGTGCGTAGTGCTCATAG
  TAATCCTCCTGGTTTGTATTACAATCATCAAGCTTATCAAGCTTTGCTTCACTTGTCACATGTTCTGTAA
  TCGGACCGTCTATGGTCCTATCAAAAACGTCTACCACATCTATCAATCATACATGCATATTGACCCATTT
  CCTAAAAGGGTCATCGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100566 representing NP_073554
  Red=Cloning sites Green=Tags
  
  MFLKLVDDHALVVNVLLWCVVLIVILLVCITIIKLIKLCFTCHMFCNRTVYGPIKNVYHIYQSYMHIDPF
  PKRVIDF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_002645
• ORF Size: 231 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_002645.1, NP_073554
• RefSeq ORF: 231
• Locus ID: 918761
• MW: 9.1 kDa