N (NC_002645) Virus Tagged ORF Clone

CAT#: VC100568

Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid protein [Human coronavirus 229E], codon optimized for human cell expression, NP_073556

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: N
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100568 represents NCBI reference of NP_073556 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACAGTGAAGTGGGCCGACGCTAGCGAGCCACAGCGAGGTAGACAAGGAAGAATTCCCTACAGCC
  TGTATTCTCCGCTGCTCGTGGATAGCGAGCAGCCTTGGAAGGTGATTCCCCGCAATCTTGTGCCAATTAA
  CAAAAAGGACAAAAATAAGCTTATCGGGTACTGGAATGTACAGAAACGGTTCAGAACACGAAAAGGAAAG
  AGAGTGGACTTGTCACCCAAACTCCACTTCTACTACCTTGGAACGGGGCCACACAAAGACGCTAAGTTCC
  GAGAGCGCGTCGAAGGCGTCGTCTGGGTGGCCGTAGATGGCGCCAAGACCGAGCCCACAGGCTATGGGGT
  AAGAAGGAAGAATTCAGAACCAGAGATCCCTCATTTCAACCAAAAACTGCCGAACGGCGTCACCGTGGTC
  GAGGAGCCCGATTCACGCGCCCCCAGTCGCTCTCAGTCTCGGAGCCAAAGCCGCGGCAGGGGGGAGAGCA
  AACCACAGAGCAGGAACCCTAGTTCAGACCGCAATCATAACTCACAGGATGATATCATGAAGGCCGTTGC
  TGCCGCTTTGAAATCTTTGGGATTTGACAAACCGCAGGAGAAGGACAAGAAGAGTGCTAAGACAGGCACG
  CCCAAGCCGTCCCGCAACCAGAGCCCTGCCTCTAGCCAAACTTCCGCCAAGAGCTTGGCTAGGAGCCAGT
  CCAGCGAAACAAAGGAGCAGAAACATGAGATGCAGAAACCAAGATGGAAACGACAGCCAAACGATGACGT
  GACATCAAACGTTACCCAATGTTTCGGCCCTCGCGACCTGGATCATAATTTCGGCTCCGCAGGAGTCGTG
  GCCAACGGTGTAAAAGCAAAAGGTTACCCCCAATTCGCTGAACTCGTTCCTAGTACTGCCGCCATGCTCT
  TCGACAGCCATATTGTTTCAAAAGAGAGCGGGAATACAGTGGTCCTGACCTTTACGACAAGAGTCACTGT
  CCCTAAGGACCATCCCCACCTGGGGAAATTTCTGGAGGAGTTGAACGCCTTTACCAGGGAGATGCAGCAA
  CACCCCCTCCTGAATCCCTCCGCGCTGGAATTTAACCCTAGTCAGACCTCACCTGCCACCGCAGAGCCTG
  TGAGGGATGAAGTGTCCATTGAGACTGACATCATCGACGAGGTAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100568 representing NP_073556
  Red=Cloning sites Green=Tags
  
  MATVKWADASEPQRGRQGRIPYSLYSPLLVDSEQPWKVIPRNLVPINKKDKNKLIGYWNVQKRFRTRKGK
  RVDLSPKLHFYYLGTGPHKDAKFRERVEGVVWVAVDGAKTEPTGYGVRRKNSEPEIPHFNQKLPNGVTVV
  EEPDSRAPSRSQSRSQSRGRGESKPQSRNPSSDRNHNSQDDIMKAVAAALKSLGFDKPQEKDKKSAKTGT
  PKPSRNQSPASSQTSAKSLARSQSSETKEQKHEMQKPRWKRQPNDDVTSNVTQCFGPRDLDHNFGSAGVV
  ANGVKAKGYPQFAELVPSTAAMLFDSHIVSKESGNTVVLTFTTRVTVPKDHPHLGKFLEELNAFTREMQQ
  HPLLNPSALEFNPSQTSPATAEPVRDEVSIETDIIDEVN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_002645
• ORF Size: 1167 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_002645.1, NP_073556
• RefSeq ORF: 1167
• Locus ID: 918763
• MW: 43.5 kDa