4b (NC_002645) Virus Tagged ORF Clone

CAT#: VC100565

Myc-DDK-tagged ORF clone of viral ORF for 4b protein [Human coronavirus 229E], codon optimized for human cell expression, NP_073553

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 4b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100565 represents NCBI reference of NP_073553 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGAAAGTGCTGGTTCCTTGAAAACAAAGCCCTGAAGCCGTTCGTGTGCTTTTACGGGGGCGACC
  AATTTCTGTACATTGGGGACAGAATCGTGTCTTACTTCAGCACTAATGACCTGTACGTGGCCCTGAGAGG
  GCGCATTGATAAAGACCTCAGCCTGTCAAGAAAGGTCGAGCTGTACAATGGTGAGTGCGTATACCTGTTC
  TGCGAACACCCTGCCGTTGGAATTGTAAACACCGATTTCAAGCTCGAGATCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100565 representing NP_073553
  Red=Cloning sites Green=Tags
  
  MQGKCWFLENKALKPFVCFYGGDQFLYIGDRIVSYFSTNDLYVALRGRIDKDLSLSRKVELYNGECVYLF
  CEHPAVGIVNTDFKLEIH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_002645
• ORF Size: 264 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_002645.1, NP_073553
• RefSeq ORF: 264
• Locus ID: 918760
• MW: 10.2 kDa