VP2 (NC_012801) Virus Tagged ORF Clone

CAT#: VC100621

Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human cosavirus B], codon optimized for human cell expression, YP_002956109

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100621 represents NCBI reference of YP_002956109 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCCAAGGTCGAAAATTCCCTGTACACTGAAGACCGGCTTCTCACTAGAAAGGCAGGCAATACCT
  CCGTCAATTCCCAGGCTGCCGAAGGAGTTTTGCAGGGCTACGGACACGAGTCCGATTTCTCCAATCCAAC
  CTCATGCGGAGATGCGCCAAGCAAAGCGGTTCCAGCTACATCAAGAGGCTATACCGTGCATCTGAAGGAT
  TGGACCAGCACCATTTATGCCTATTATGCGCAACTGTACAGGATTTCTGACAGGATTAAGGACCCAGCCA
  TCGGAAACCTCTTTTCCAGGAACATGGATGCGCACTCCTTCACAAAGACTGGCTACGAGGTTATGCTGCA
  AGTAAATACATCCCCCTTCCACTCTGGACTGATCGGCCTCTTCCTTGTTCCTGAACTTGTGAGAAGCACC
  GGCTCTGATCTGGAATGGATGGTGATGACCCAGAAGCTCTCCCTGTACCAACCTCCTGCCGAAGAAATAG
  CGCATAGTCACTATTCCACTCAGACGATTACTCCCAATAAGAATGTGTCTTTTGACCTGGCAGACATGAC
  CGCCGAGCAGATGATGCTGTTTCCCCATCAGCTGATTAATCCTAAGGACACAAACGTGGCCACCGTGAGA
  GTGCCCTACGTAAATGTTGCCCCTACTAGCGATCCCCGGGTGCATAACATCTGGACAGCCGTAATCATGG
  TTGTGACGCCCCTCAAGTACGCCGACGGAGCGTCCCCCACCGTGCAGATGACACTCACCATAACTCCGAT
  CGACACCGTATTCAACGGTCTGCATCACGCATCTTCTACTGCACAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100621 representing YP_002956109
  Red=Cloning sites Green=Tags
  
  MSPKVENSLYTEDRLLTRKAGNTSVNSQAAEGVLQGYGHESDFSNPTSCGDAPSKAVPATSRGYTVHLKD
  WTSTIYAYYAQLYRISDRIKDPAIGNLFSRNMDAHSFTKTGYEVMLQVNTSPFHSGLIGLFLVPELVRST
  GSDLEWMVMTQKLSLYQPPAEEIAHSHYSTQTITPNKNVSFDLADMTAEQMMLFPHQLINPKDTNVATVR
  VPYVNVAPTSDPRVHNIWTAVIMVVTPLKYADGASPTVQMTLTITPIDTVFNGLHHASSTAQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012801
• ORF Size: 816 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012801.1, YP_002956109
• RefSeq ORF: 816
• MW: 30.0 kDa