VP1 (NC_012801) Virus Tagged ORF Clone

CAT#: VC100623

Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human cosavirus B], codon optimized for human cell expression, YP_002956111

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100623 represents NCBI reference of YP_002956111 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCCAGGGTCCACACCACATGACAATGCCGAGAAAGGGACAATAGAAAATTGCGATGCGAGTCTGA
  ATTCTGGACACAGCGTCAGCTTGCCCACCCCGCATAGTAATGTTAGATTTTTTTTTGACAGGTATAGGTT
  TCTTGGCCTCTTGGAGTCCACCCCTAATACGGCGCCAGAAATCACTTCACCATACGACCCAACATCCTTC
  AAGGTTAGGGACCTGGCCTATATGTTCAACGTGAACAACACGAAAAGGCCTTTTAATGCCCTTGCTCTCA
  CACCCACTCCCAGCATCGACGGCAGTCCCATTACCACATACCTTGTGTCCTCTAACATGGCCCAGAGCGA
  CTACAACTACATTTTCACGCCTGGAGATCCCCACCTGTATCGGAGTTGTCCTTTTACCTACTTCCACGCC
  GATTTGGAGGTTACAGTGAAGCCTCCGCCCGAGCTCACAGGGCGATGGCGCGTGACATGGTATCCCCCAG
  GGGCAACCATCGACACCGTTACGGTGCACCTCGCTACAGAGGCGTCCACGGAAAGTTCAACAACCACAGT
  GACCAGTAGCCTCTCCAGTTCTGGATCATTGTTTACCCTGAATCCTACTTTTTACGGCAAAAATGGCTTC
  CCCGTTTCTTTCATGATCCCATTCTGCAGTCCGCTGACTCTGCTGCCACTGTACTTTGATGGCTATCCCG
  ATTACAAGCGATCTAGTGGGGCATACGGGATCGGACCTGCCAGCACTTTTGGAACCCTTACTGTGGACTA
  TGATGGCACCAAACAATTCTTTTCTGTGTTCATTAGGTATAAAAACTTTAGGGGTTACATCCCCAGGCCC
  GTGATACGGTTCCCAACGACAAACCCAGATGCCAATGTGAAATACATTACCCTGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100623 representing YP_002956111
  Red=Cloning sites Green=Tags
  
  MSPGSTPHDNAEKGTIENCDASLNSGHSVSLPTPHSNVRFFFDRYRFLGLLESTPNTAPEITSPYDPTSF
  KVRDLAYMFNVNNTKRPFNALALTPTPSIDGSPITTYLVSSNMAQSDYNYIFTPGDPHLYRSCPFTYFHA
  DLEVTVKPPPELTGRWRVTWYPPGATIDTVTVHLATEASTESSTTTVTSSLSSSGSLFTLNPTFYGKNGF
  PVSFMIPFCSPLTLLPLYFDGYPDYKRSSGAYGIGPASTFGTLTVDYDGTKQFFSVFIRYKNFRGYIPRP
  VIRFPTTNPDANVKYITLE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012801
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012801.1, YP_002956111
• RefSeq ORF: 897
• MW: 33.2 kDa