2B (NC_012801) Virus Tagged ORF Clone

CAT#: VC100625

Myc-DDK-tagged ORF clone of viral ORF for 2B [Human cosavirus B], codon optimized for human cell expression, YP_002956113

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100625 represents NCBI reference of YP_002956113 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGTGCAAAGTAAGTATGAGGCTCAAGGACCTATCGACCTGCTGCAGCTTCTCAGGAACCCTCAAA
  CCGTCGATAATGTGACAAGACTCTTGAACACCCTTAACAATTTGATGCAAACCTGGAATAACATCAAGAC
  CACTCTGTCTGATGCCATTTTTCTGCGCGACATGGTATGCCTGCTGGTGAAGCTGACAAGTCTGGCCTAC
  CTGGTTAAGGACCAGGGACCAGGAGCCTACTTCGCCGCCGCTGCGATCCTGGTGTCCGATGGAATCTCCT
  TTTTGGATTGGTATGAGAAAATCAGGATTTTCATGAGTAGGAGGCTTAGGACCCCCCCTCCCCCCATGTT
  TAAAGTTCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100625 representing YP_002956113
  Red=Cloning sites Green=Tags
  
  MPVQSKYEAQGPIDLLQLLRNPQTVDNVTRLLNTLNNLMQTWNNIKTTLSDAIFLRDMVCLLVKLTSLAY
  LVKDQGPGAYFAAAAILVSDGISFLDWYEKIRIFMSRRLRTPPPPMFKVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012801
• ORF Size: 360 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012801.1, YP_002956113
• RefSeq ORF: 360
• MW: 13.7 kDa