3C (NC_012801) Virus Tagged ORF Clone

CAT#: VC100626

Myc-DDK-tagged ORF clone of viral ORF for 3C [Human cosavirus B], codon optimized for human cell expression, YP_002956114

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100626 represents NCBI reference of YP_002956114 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCCGATCTGCGAGACGTGGTGACTTTCTTCAACGCAGCCAAAGGAGCCCAGTGGATGGTGGAGT
  CCATCAAGAGCCTGATAAACTGGATTAAGCAGTGGCTGGAGTTGGAAGAGGAGAACGAGGCAGTTAAGCT
  GGAGAAAATGCTGATCGAGTCACCATCACACTGTAAAAACATACACATGTACAACAAGGGCGAATTGTTC
  GCCAAACCTCAAGAGTCTTTCGACTTCCTGGACCGGCTGTGCGAGACAGCTACAAGCCTCGGTAAGACAC
  ACCTGGCATCTTACTTCCGCAATTTCGTGACATACGACTCAGATACATCTCGCCCTGAACCAGTCGTAGT
  TGTGTTGAGGGGTAAGCCCGGAGCCGGCAAATCAGCCGCTGCCACCGTCTTGGCTGCAGCAGTATCAAAG
  CTGTGTGTCGGCAGTCAATCAGTCTACACATTGTCCCCCGACACAGAACATATGGACGGCTATCACGGGC
  AGTTCGCAACCATCATGGATGATCTGGGCCAGAACCCTGACGGTGAAGATTTCCGCTCTTTCTGTCAGAT
  GGTTAGCTGCGCCCAGTACCGGCCGCCTATGGCAGGTCTCCCAGATAAGGGTATACTCTTTACCTCCCGG
  ATTGTGATCGCGACCACCAATCTTTGCGACTTTAACCCGTTGACTATAGCCGACCCTAGAGCCCTGGAGC
  GGCGCATCACCTTTGATATTCTGGTCTCACCAGGTCCTGCCTGCAACAAAAATGGAAAACTCGACCTGAA
  CGCTGCCCTGACACCTGATGGACCAGGTGAAGGGCCATTCACAACTGATTGCCAAATTCTGCACACAACA
  GGACTGACTCTGAAGGATATCCGCAAAGGAACCACACTGAACCTCAAGGACCTGACAGAACTGGTGGTTG
  ATAGAATTAACAAGAAGAAGAAGGTGGGGAACATGCTTGAGAACCTGGTCGCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100626 representing YP_002956114
  Red=Cloning sites Green=Tags
  
  MGPDLRDVVTFFNAAKGAQWMVESIKSLINWIKQWLELEEENEAVKLEKMLIESPSHCKNIHMYNKGELF
  AKPQESFDFLDRLCETATSLGKTHLASYFRNFVTYDSDTSRPEPVVVVLRGKPGAGKSAAATVLAAAVSK
  LCVGSQSVYTLSPDTEHMDGYHGQFATIMDDLGQNPDGEDFRSFCQMVSCAQYRPPMAGLPDKGILFTSR
  IVIATTNLCDFNPLTIADPRALERRITFDILVSPGPACNKNGKLDLNAALTPDGPGEGPFTTDCQILHTT
  GLTLKDIRKGTTLNLKDLTELVVDRINKKKKVGNMLENLVAQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012801
• ORF Size: 966 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012801.1, YP_002956114
• RefSeq ORF: 966
• MW: 35.5 kDa