3C (NC_012801) Virus Tagged ORF Clone

CAT#: VC100629

Myc-DDK-tagged ORF clone of viral ORF for 3C [Human cosavirus B], codon optimized for human cell expression, YP_002956117

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100629 represents NCBI reference of YP_002956117 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACCCATGCTCGATCTGGAAAAAAAGGTGTTCAAAAACGTGCTGCCTTTTGGCCTCGTGTATAATG
  GGAAGACATATCAGCAGAGTTGTCTGGCTATTGGTAAAAGGGTCATTCTCGTAAACGCCCATGCGTTCAA
  CTCCATTGAGGATCGCTTTGAAGTGGACGGCAAAACTTATCACCTGGACGATGTGGACGTGTGTGTCCTG
  GACACCAGTGAAGGTCTCAGCGATGTGTGCGCAGTGAAACTGCCCCCCGGCCCTGACTTCCCCAGCGTGG
  TGCGCCTGTTCATGCCGTTCCATGCTGATCTGCATCCCGGGCTGCGCCTGACCATCCTGAGCAATGACCA
  GCTCCCAATGATGAGGGAGGGCTCCTTTCTTAGAAATGAGGATTACGCTCCCACCAACATAGGGAATATC
  CCATTTGTCTGCTTGTACCGATCTACCTCTTACTTTGGCATGTGCGGCAGCCCTGTCATGACCCGCGGGC
  CTGATTCCCAGGGAATTCTTGGAATACACTGTGCAGGTGGGGGCGGTGTGTCAGTGTTGTCAAGAGTTAC
  CAGGAGAATGGCTGAGAGCGTGATAGATTACTTTTATCCGCTGCAGACACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100629 representing YP_002956117
  Red=Cloning sites Green=Tags
  
  MSPMLDLEKKVFKNVLPFGLVYNGKTYQQSCLAIGKRVILVNAHAFNSIEDRFEVDGKTYHLDDVDVCVL
  DTSEGLSDVCAVKLPPGPDFPSVVRLFMPFHADLHPGLRLTILSNDQLPMMREGSFLRNEDYAPTNIGNI
  PFVCLYRSTSYFGMCGSPVMTRGPDSQGILGIHCAGGGGVSVLSRVTRRMAESVIDYFYPLQTQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012801
• ORF Size: 612 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012801.1, YP_002956117
• RefSeq ORF: 612
• MW: 22.6 kDa