VP3 (NC_012801) Virus Tagged ORF Clone

CAT#: VC100622

Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human cosavirus B], codon optimized for human cell expression, YP_002956110

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100622 represents NCBI reference of YP_002956110 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCCTATACCCACCCGGCCTTTCCACCACTCATTCCAATTTAGTAGCACTGTGCCAGACGTCACCG
  AGCCATGCTACGGTATGACAGTGAATCCTCCAAAAGATTATATGCCCGAACCTGTGGGGGATCTCGTCTC
  CCTGGCGAAGGTTGGTTCCCTGGTGACTGTCTCCTACCCCAGCAACTCTGATCAACCCTACTTCGAAATC
  AGCAATACTAAGAAGGATACGCCACTGTTCAAGTGTAATGTACTGTTGTCCGACCCACATTTTCAACATA
  CCCTGGTCTCTAATCTCGGCAAGTACTTCGCAAATTACAGAGGCAGCTTGCAATTCTCATTCATATCCGC
  TACTACCGGCATGACAAAAGGGAAGCTTCTGCTCAGTTACACACCCCCGGGAAGCGGAGCTCCCACCACG
  CTGGAACAGGCAATGAACGGAACTTACTCCATCTGGGATATTGGCTTGCAGAGTACCTTCAACTTTGTGG
  TGCCTTACATTTCCGCTGTCGATTTCCGCCTTTGCGCCGCCAGCGCGTCTAGTGTCGTCAACACCGACGG
  ATGGTTCACCGTTTGGCTTCTGAATTCACTCACCTACCCGCCCGGGACCCCACCTACCCAGAACGTGGTA
  GTGATGCTTTCCGCCGGTGAGGACTTTAGTTACCGCAATCCAATCTCCCCAGCCTGGACCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100622 representing YP_002956110
  Red=Cloning sites Green=Tags
  
  MSPIPTRPFHHSFQFSSTVPDVTEPCYGMTVNPPKDYMPEPVGDLVSLAKVGSLVTVSYPSNSDQPYFEI
  SNTKKDTPLFKCNVLLSDPHFQHTLVSNLGKYFANYRGSLQFSFISATTGMTKGKLLLSYTPPGSGAPTT
  LEQAMNGTYSIWDIGLQSTFNFVVPYISAVDFRLCAASASSVVNTDGWFTVWLLNSLTYPPGTPPTQNVV
  VMLSAGEDFSYRNPISPAWTQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012801
• ORF Size: 693 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012801.1, YP_002956110
• RefSeq ORF: 693
• MW: 25.3 kDa