32 kD non-structural protein (NC_012950) Virus Tagged ORF Clone

CAT#: VC100657

Myc-DDK-tagged ORF clone of viral ORF for 32 kD non-structural protein [Human enteric coronavirus strain 4408], codon optimized for human cell expression, YP_003038520

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 32 kD non-structural protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100657 represents NCBI reference of YP_003038520 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGGCGTATGCTGACAAACCTAATCACTTCATCAACTTTCCCCTGACCCAGTTTGAGGGGTTTG
  TGCTCAATTATAAAGGCCTGCAGTTTCAGCTGCTGGACGAGGGGGTAGACTGCAAAATTCAGACTGCACC
  GCACATTTCTCTCGCTATGTTGGATATCCAACCTNNNGATTACCGCTCAGTGGATGTGGCGATACAGGAG
  GTGATTGATGATATGCATTGGGGAGAAGGTTTTCAGATCNNNTTCNNNAACCCCCATATACTCGGACGAT
  GCATTGTGCTCGACGTGAAGGGAGTCGAGGAACTTCACGATGATTTGGTGAACTACATCCGCGATAAAGG
  CTGCGTGGCAGATCAAAGTCGGAAATGGATCGGCCATTGTACAATCGCTCAGCTTACANNNGCAGCACTG
  AGTATTAAAGAAAATGTAGACTTCATCAACAGTATGCAGTTTAACTATAAAATCACCATAAATCCATCAT
  CACCCGCGAGACTGGAGATAGTTAAACTTGGCGCCGAAAAGAAAGATGGTTTCTATGAGACTATCGTGTC
  TCATTGGATGGGAATTCGGTTTGAGTATAACCCACCAACAGACAAGCTGGCAATGATCATGGGATACTGC
  TGTCTGNNNGTAGTGCGCAAGGAGCTTGAGGAGGGTGACCTGCCAGAGAATGACGACGACGCTTGGTTTA
  AGCTGTCTTACCATTATGAGAATAACTCCTGGTTCTTTCGGCATGTGTACAGGAAGAGCTCTTACTTCCG
  AAAGAGCTGCCAGAATCTGGACTGTAACTGTTTGGGATTCTACGAGAGCTCCGTAGAAGAGNNN
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100657 representing YP_003038520
  Red=Cloning sites Green=Tags
  
  MAVAYADKPNHFINFPLTQFEGFVLNYKGLQFQLLDEGVDCKIQTAPHISLAMLDIQPXDYRSVDVAIQE
  VIDDMHWGEGFQIXFXNPHILGRCIVLDVKGVEELHDDLVNYIRDKGCVADQSRKWIGHCTIAQLTXAAL
  SIKENVDFINSMQFNYKITINPSSPARLEIVKLGAEKKDGFYETIVSHWMGIRFEYNPPTDKLAMIMGYC
  CLXVVRKELEEGDLPENDDDAWFKLSYHYENNSWFFRHVYRKSSYFRKSCQNLDCNCLGFYESSVEEX
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012950
• ORF Size: 834 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012950.1, YP_003038520
• RefSeq ORF: 834
• MW: 32.2 kDa