envelope protein (NC_012950) Virus Tagged ORF Clone

CAT#: VC100663

Myc-DDK-tagged ORF clone of viral ORF for envelope protein [Human enteric coronavirus strain 4408], codon optimized for human cell expression, YP_003038526

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: envelope protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100663 represents NCBI reference of YP_003038526 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTATGGCTGACGCCTACTTTGCTGATACCGTTTGGTACGTTGGACAAATCATCTTTATAGTGGCAA
  TCTGTTTGTTGGTTATCATCGTCGTGGTTGCCTTTCTGGCCACATTTAAACTCTGTATTCAGCTGTGCGG
  GATGTGTAATACCCTCGTTCTCTCACCTTCAATCTATGTCTTCAATCGCGGAAGGCAGTTTTATGAGTTT
  TATAATGACGTGAAGCCCCCAGTACTCGATGTGGATGACGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100663 representing YP_003038526
  Red=Cloning sites Green=Tags
  
  MFMADAYFADTVWYVGQIIFIVAICLLVIIVVVAFLATFKLCIQLCGMCNTLVLSPSIYVFNRGRQFYEF
  YNDVKPPVLDVDDV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012950
• ORF Size: 252 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012950.1, YP_003038526
• RefSeq ORF: 252
• MW: 9.6 kDa