nucleocapsid protein (NC_012950) Virus Tagged ORF Clone

CAT#: VC100665

Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid protein [Human enteric coronavirus strain 4408], codon optimized for human cell expression, YP_003038528

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nucleocapsid protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100665 represents NCBI reference of YP_003038528 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTTTACTCCCGGAAAACAGAGCTCCTCTCGGGCAAGTAGCGGGAATCGGTCAGGGAATGGAATCC
  TGAAGTGGGCCGACCAGTCTGATCAGTCCAGGAACGTGCAGACCCGCGGTAGAAGAGCCCAGCCCAAACA
  GACAGCGACGTCACAGCAGCCCTCAGGCGGGAACGTTGTGCCATATTACAGCTGGTTTAGCGGTATAACT
  CAGTTCCAGAAAGGTAAGGAGTTTGAATTCGCCGAAGGCCAAGGCGTTCCTATAGCTCCCGGAGTGCCAG
  CCACTGAGGCAAAGGGTTACTGGTACAGGCACAATAGAAGGTCTTTCAAAACCGCCGACGGCAATCAGCG
  ACAGCTGCTCCCTCGGTGGTATTTCTATTACCTGGGAACCGGTCCTCACGCCAAGGACCAGTATGGCACG
  GACATCGATGGCGTGTTCTGGGTGGCCAGTAATCAGGCCGATGTCAACACTCCGGCCGACATTTTGGATA
  GAGACCCAAGTAGCGATGAGGCTATCCCAACGCGCTTTCCACCCGGGACAGTTCTCCCGCAGGGATATTA
  CATTGAAGGCAGCGGGCGGTCCGCCCCTAATTCTCGATCCACCTCTCGAGCATCCTCACGGGCGAGCTCC
  GCTGGGAGCCGCTCCCGGGCCAACTCCNNNAATCGCACACCAACTTCCGGCGTGACCCCGGACATGGCTG
  ATCAAATTGCCTCACTGGTGTTGGCCAAACTCGGAAAAGACGCTACCAAGCCACAACAGGTGACTAAACA
  AACTGCAAAGGAGATAAGGCAGAAGATTCTCAATAAACCCCGCCAGAAACGGTCCCCTAACAAGCAATGC
  ACGGTTCAGCAATGCTTCGGGAAACGCGGTCCAAACCAAAACTTCGGCGGAGGTGAAATGCTTAAATTGG
  GCACGTCTGACCCACAGTTCCCCATCCTGGCCGAGCTGGCGCCCACTGCCGGAGCCTTCTTCTTTGGCTC
  CAGACTGGAACTGGCCAAGGTGCAGAACCTTTCAGGAAATTTGGATGAACCGCAAAAAGATGTTTATGAA
  CTTCGATATNNNGGTGCCATTCGATTTGACAGTACTCTCTCTGGCTTTGAAACCATCATGAAAGTTCTGA
  ATGAAAATCTTAACGCCTATCAACAGCAGGATGGAATGATGAACATGTCACCGAAACCACAGCGGCAGAG
  AGGCCAGAAGAACGGCCAGGGTGAGAACGACAATATTAGCGTAGCCGCTCCTAAGTCCAGGGTCCAACAG
  AACAAGTCAAGAGAATTGACCGCGGAGGACATTTCATTGCTGAAGAAGATGGACGAGCCATATACAGAGG
  ACACCTCCGAAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100665 representing YP_003038528
  Red=Cloning sites Green=Tags
  
  MSFTPGKQSSSRASSGNRSGNGILKWADQSDQSRNVQTRGRRAQPKQTATSQQPSGGNVVPYYSWFSGIT
  QFQKGKEFEFAEGQGVPIAPGVPATEAKGYWYRHNRRSFKTADGNQRQLLPRWYFYYLGTGPHAKDQYGT
  DIDGVFWVASNQADVNTPADILDRDPSSDEAIPTRFPPGTVLPQGYYIEGSGRSAPNSRSTSRASSRASS
  AGSRSRANSXNRTPTSGVTPDMADQIASLVLAKLGKDATKPQQVTKQTAKEIRQKILNKPRQKRSPNKQC
  TVQQCFGKRGPNQNFGGGEMLKLGTSDPQFPILAELAPTAGAFFFGSRLELAKVQNLSGNLDEPQKDVYE
  LRYXGAIRFDSTLSGFETIMKVLNENLNAYQQQDGMMNMSPKPQRQRGQKNGQGENDNISVAAPKSRVQQ
  NKSRELTAEDISLLKKMDEPYTEDTSEI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012950
• ORF Size: 1344 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012950.1, YP_003038528
• RefSeq ORF: 1344
• MW: 49.4 kDa