spike protein (NC_012950) Virus Tagged ORF Clone

CAT#: VC100659

Myc-DDK-tagged ORF clone of viral ORF for spike protein [Human enteric coronavirus strain 4408], codon optimized for human cell expression, YP_003038522

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: spike protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100659 represents NCBI reference of YP_003038522 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCTGATTCTCCTGATCTCTCTCCCGATGGCACTTGCTGTAATCGGCGATCTTAAGTGCACTACGG
  TGAGTATCAACGATGTAGATACCGGCGTCCCGTCTATCTCTACAGACACCGTCGACGTTACGAATGGACT
  GGGTACTTACTACGTCCTTGATCGAGTCTACCTTAACACCACATTGCTGCTGAACGGCTACTACCCTACA
  TCAGGATCAACCTATCGGAACATGGCGCTGAAAGGGACACTCCTCCTGTCTACTCTCTGGTTTAAGCCCC
  CCTTCCTGAGCGACTTCACCAACGGTATTTTCGCTAAGGTGAAGAATACGAAGGTCACTAAAAACGGTCT
  GATGTACAGTGAATTTCCCGCTATTACCATTGGGTCTACATTCGTGAACACTAGCTACAGCGTTGTTGTC
  CAACCACATACGACTAATCTTGATAACAAGCTGCAGGGTTTGCTCGAAATCAGTGTGTGTCAGTACACCA
  TGTGCGAATATCCGAATACGATCTGTCACCCAAACCTTGGAAATCGGCGGGTAGAACTGTGGCACTGGGA
  TACAGGCGTGGTGTCCTGCCTTTATAAGAGGAACTTCACCTATGATGTCAACGCAGATTACCTGTATTTT
  CATTTCTACCAGGAGGGGGGGACTTTCTACGCTTATTTCACTGACACGGGTGTCGTAACCAAGTTTCTCT
  TTAATGTGTATCTCGGAACCGTGCTCTCCCACTACTACGTGATGCCACTGACATGCAACAGCGCCATGTC
  CCTGGAATATTGGGTCACCCCACTCACCTCTAAGCAGTATCTCTTGGCTTTTAACCAGGACGGCGTCATA
  TTTAACGCGGTGGACTGCAAGAGCGATTTCATGAGCGAAATTAAGTGTAAAACTCTGTCTATAGCACCTA
  GCACCGGAGTATATGAGCTGAACGGGTACACCGTGCAGCCTATCGCAGATGTTTATAGACGCATTCCAAA
  TCTCCCAGATTGCAATATTGAGGCCTGGCTGAATGACAAGAGCGTGCCAAGTCCTCTCAACTGGGAACGG
  AAGACCTTTTCCAATTGTAACTTCAACATGAGCAGTCTGATGTCTTTTATCCAGGCAGACAGCTTCACCT
  GCAACAATATCGATGCTGCTAAGATTTACGGTATGTGTTTTAGTAGCATCACCATTGATAAGTTCGCCAT
  CCCTAACTCTAGGAAAGTCGACCTGCAACTTGGCAATCTGGGTTACCTGCAGAGTTTTAATTATAGAATC
  GATACCACTGCGACCTCCTGCCAACTTTACTATAATCTGCCCGCCGCCAATGTCTCAGTGTCAAGATTCA
  ACCCTAGCACCTGGAATCGCCGATTTGGGTTTACAGAGCAGTCCGTGTTTAAGCCCCAGCCAGCCGGGGT
  CTTTACTGATCATGACGTGGTATACGCTCAGCACTGTTTCAAGGCACCCACAAATTTCTGCCCGTGCAAG
  CTGGATGGCAGTCTGTGTGTAGGAAACGGCCCCGGCATTGACGCTGGTTACAAAAATAGTGGGATCGGAA
  CGTGTCCCGCGGGAACTAATTACCTTACCTGTCACAATGCCGCACAATGTAACTGCCTGTGTACACCGGA
  CCCTATTACTAGCAAGAGCACTGGACCTTATAAATGTCCGCAGACAAAGTACCTTGTTGGCATCGGCGAA
  CATTGCAGTGGACTGGCGATTAAATCAGATCACTGCGGGGGGAATCCTTGCACCTGTCAGCCTCAGGCAT
  TTCTGGGATGGAGCGTGGAGTCCTGTCTCCAGGGGGATCGGTGCAACATTTTTGCAAATTTTATCTTGCA
  CGGAGTGAATTCTGGAACCACATGCTCCACTGACCTGCAGAAGTCAAATACAGACATAATTCTTGGCGTG
  TGTGTGAATTATGATCTTTACGGTATTACAGGCCAGGGGATCTTTGTGGAGGTAAATGCTACCTATTACA
  ACTCATGGCAAAACCTGCTGTACGATTCAAATGGAAATTTGTATGGATTCCGGGATTACCTTACTAATCG
  GACTTTCATGATCAGGAGCTGTTACTCCGGGCGGGTGAGCGCTGCATTCCATGCCAACAGCAGTGAGCCG
  GCTCTCCTCTTCCGCAATATCAAGTGTAATTACGTATTTAATAACACTCTGTCCCGGCAGCTCCAGCCCA
  TCAATTACTTCGACTCTTACCTTGGTTGCGTGGTCAATGCCGATAACTCAACGAGCTCAGTCGTACAGAC
  ATGCGACCTCACAGTGGGCTCAGGCTACTGCGTCGATTATTCCACCAAACGCAGGTCCCGGAGAGCTATC
  ACCACCGGATATAGGTTTACAAACTTTGAGCCTTTTACTGTGAACAGTGTGAATGATAGTCTTGAGCCTG
  TGGGCGGCCTGTATGAGATTCAAATTCCTTCTGAATTCACCATAGGCAACATGGAAGAGTTCATTCAAAC
  GTCTTCACCCAAGGTCACCATTGACTGTTCAGCCTTCGTTTGCGGCGACTATGCGGCTTGCAAGTCACAA
  CTCGTTGAGTACGGGAGTTTCTGCGACAATATCAATGCCATCCTTACTGAAGTGAACGAGCTCCTGGACA
  CAACTCAGCTTCAGGTGGCTAACTCACTGATGAACGGCGTCACCCTGAGCACCAAGTTGAAGGATGGAGT
  CAATTTCAACGTCGATGACATTAATTTTTCCCCCGTGCTGGGCTGCCTCGGGTCCGATTGCAACCGGGTT
  TCCAGCAGGTCCGCCATCGAAGATCTGCTCTTCAGCAAGGTCAAGTTGGCTGATGTCGGGTTCGTTGAGG
  CCTACAATAATTGCACCGGGGGGGCTGAAATTCGGGATCTGATCTGCGTGCAGAGCTATAACGGAATAAA
  AGTGCTGCCTCCACTGCTTTCTGAGAATCAGATCAGCGGGTATACTTTGGCCGCCACATCAGCATCACTC
  TTTCCTCCTTGGAGCGCAGCAGCTGGTGTGCCTTTTTATCTCAATGTCCAGTATAGAATTAATGGAATCG
  GTGTGACCATGGATGTCCTCTCACAAAATCAAAAGCTGATTGCGAACGCCTTCAACAACGCTCTGGGCGC
  CATTCAGGAGGGTTTCGATGCAACCAACTCAGCACTGGTGAAGATACAGGCCGTCGTCAATGCAAATGCA
  GAAGCCCTGAATAACCTCCTTCAACAGCTCTCAAACCGATTCGGTGCCATCTCTTCCTCATTGCAGGAAA
  TTCTGAGTCGGCTTGACGCTCTGGAGGCCCAGGCTCAGATCGACAGACTTATTAATGGGAGACTCACGGC
  CTTGAACGCCTACGTTTCTCAGCAGCTGAGCGATAGTACCCTCGTGAAGTTCTCCGCTGCACAGGCAATG
  GAAAAAGTGAACGAGTGCGTAAAGAGTCAGAGCTCTCGCATTAATTTTTGTGGCAACGGTAATCACATAA
  TCTCACTGGTGCAGAACGCCCCATATGGCCTGTACTTCATCCACTTTTCTTATGTACCGACCAAATACGT
  CACAGCAAAAGTGAGCCCCGGCCTGTGCATTGCGGGCGACCGGGGTATAGCACCTAAGTCTGGCTATTTT
  GTGAATGTCAATAACACATGGATGTTCACAGGTTCAGGATACTACTATCCGGAACCAATTACTGGCAATA
  ACGTGGTCGTTATGAGTACATGCGCGGTAAATTATACAAAGGCTCCAGATGTGATGTTGAATATTTCTAC
  CCCGAATCTGCCCGATTTCAAGGAGGAGTTGGACCAGTGGTTTAAAAATCAGACCAGTGTGGCTCCCGAT
  CTTAGCCTCGACTATATTAATGTGACATTCCTGGATCTTCAAGAGGAGATGAACCGACTTCAGGAGGCGA
  TTAAAGTACTCAATCACTCCTACATCAATCTGAAAGACATTGGAACTTACGAGTATTATGTAAAATGGCC
  CTGGTATGTATGGCTGCTGATCGGATTCGCCGGGGTGGCCATGCTCGTGCTCCTGTTTTTTATCTGTTGC
  TGTACCGGCTGTGGAACAAGTTGCTTCAAAAAATGCGGCGGATGCTGTGATGACTATACAGGGCACCAGG
  AGCTGGTGATTAAGACCAGCCACGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100659 representing YP_003038522
  Red=Cloning sites Green=Tags
  
  MFLILLISLPMALAVIGDLKCTTVSINDVDTGVPSISTDTVDVTNGLGTYYVLDRVYLNTTLLLNGYYPT
  SGSTYRNMALKGTLLLSTLWFKPPFLSDFTNGIFAKVKNTKVTKNGLMYSEFPAITIGSTFVNTSYSVVV
  QPHTTNLDNKLQGLLEISVCQYTMCEYPNTICHPNLGNRRVELWHWDTGVVSCLYKRNFTYDVNADYLYF
  HFYQEGGTFYAYFTDTGVVTKFLFNVYLGTVLSHYYVMPLTCNSAMSLEYWVTPLTSKQYLLAFNQDGVI
  FNAVDCKSDFMSEIKCKTLSIAPSTGVYELNGYTVQPIADVYRRIPNLPDCNIEAWLNDKSVPSPLNWER
  KTFSNCNFNMSSLMSFIQADSFTCNNIDAAKIYGMCFSSITIDKFAIPNSRKVDLQLGNLGYLQSFNYRI
  DTTATSCQLYYNLPAANVSVSRFNPSTWNRRFGFTEQSVFKPQPAGVFTDHDVVYAQHCFKAPTNFCPCK
  LDGSLCVGNGPGIDAGYKNSGIGTCPAGTNYLTCHNAAQCNCLCTPDPITSKSTGPYKCPQTKYLVGIGE
  HCSGLAIKSDHCGGNPCTCQPQAFLGWSVESCLQGDRCNIFANFILHGVNSGTTCSTDLQKSNTDIILGV
  CVNYDLYGITGQGIFVEVNATYYNSWQNLLYDSNGNLYGFRDYLTNRTFMIRSCYSGRVSAAFHANSSEP
  ALLFRNIKCNYVFNNTLSRQLQPINYFDSYLGCVVNADNSTSSVVQTCDLTVGSGYCVDYSTKRRSRRAI
  TTGYRFTNFEPFTVNSVNDSLEPVGGLYEIQIPSEFTIGNMEEFIQTSSPKVTIDCSAFVCGDYAACKSQ
  LVEYGSFCDNINAILTEVNELLDTTQLQVANSLMNGVTLSTKLKDGVNFNVDDINFSPVLGCLGSDCNRV
  SSRSAIEDLLFSKVKLADVGFVEAYNNCTGGAEIRDLICVQSYNGIKVLPPLLSENQISGYTLAATSASL
  FPPWSAAAGVPFYLNVQYRINGIGVTMDVLSQNQKLIANAFNNALGAIQEGFDATNSALVKIQAVVNANA
  EALNNLLQQLSNRFGAISSSLQEILSRLDALEAQAQIDRLINGRLTALNAYVSQQLSDSTLVKFSAAQAM
  EKVNECVKSQSSRINFCGNGNHIISLVQNAPYGLYFIHFSYVPTKYVTAKVSPGLCIAGDRGIAPKSGYF
  VNVNNTWMFTGSGYYYPEPITGNNVVVMSTCAVNYTKAPDVMLNISTPNLPDFKEELDQWFKNQTSVAPD
  LSLDYINVTFLDLQEEMNRLQEAIKVLNHSYINLKDIGTYEYYVKWPWYVWLLIGFAGVAMLVLLFFICC
  CTGCGTSCFKKCGGCCDDYTGHQELVIKTSHDD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012950
• ORF Size: 4089 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012950.1, YP_003038522
• RefSeq ORF: 4089
• MW: 150.6 kDa