hemagglutinin-esterase (NC_012950) Virus Tagged ORF Clone

CAT#: VC100658

Myc-DDK-tagged ORF clone of viral ORF for hemagglutinin-esterase [Human enteric coronavirus strain 4408], codon optimized for human cell expression, YP_003038521

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hemagglutinin-esterase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100658 represents NCBI reference of YP_003038521 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTTGTTGCCCCGATTCGTGCTGGTAAGCTGCATCATTGGTAGCCTGGGTTTCGAGAACCCCCCCA
  CCAATGTCGTCAGCCACCTCAATGGGGACTGGTTTCTGTTCGGCGATTCCAGGTCTGACTGCAATCACGT
  GGTGAACACTAACCCGAGGAATTATAGCTACATGGACCTCAACCCCGCACTGTGTGATAGCGGGAAAATT
  AGCTCAAAGGCCGGAAATTCCATCTTCCGCTCTTTCCATTTCACCGACTTCTACAACTACACGGGAGAGG
  GCCAGCAGATAATTTTTTACGAGGGCGTTAACTTCACCCCCTACCACGCATTCAAATGCACTACATCTGG
  GTCTAATGATATATGGATGCAAAACAAGGGGCTGTTCTATACCCAGGTTTACAAGAACATGGCCGTATAT
  AGAAGCCTCACCTTCGTAAATGTACCATATGTCTACAATGGAAGCGCCCAAAGCACAGCTCTCTGTAAGT
  CTGGCTCACTGGTTCTGAACAATCCAGCATACATCGCCAGGGAAGCAAACTTTGGAGATTATTACTATAA
  GGTCGAAGCAGACTTCTACCTGTCAGGGTGTGATGAATATATCGTTCCTCTCTGTATCTTCAACGGAAAG
  TTCCTCTCTAATACTAAATACTATGACGACAGTCAGTACTATTTCAACAAAGACACAGGAGTTATTTACG
  GCCTGAATAGTACAGAGACTATTACCACAGGATTTGATTTCAATTGCCACTATTTGGTGTTGCCGTCAGG
  GAATTACTTGGCTATTAGCAATGAGCTTCTGCTGACAGTACCCACCAAAGCCATCTGTCTGAATAAAAGA
  AAAGACTTTACACCAGTACAAGTCGTGGACTCTCGCTGGAATAATGCGAGGCAGAGTGATAATATGACAG
  CAGTAGCTTGCCAGCCTCCATATTGTTACTTTAGAAACTCTACTACAAATTACGTTGGGGTCTATGATAT
  TAATCACGGAGACGCGGGCTTCACCAGCATCCTTTCCGGGCTTCTTTATGATTCCCCATGTTTTTCTCAG
  CAGGGAGTGTTCAGATATAATAACGTGAGCTCAGTTTGGCCATTGTACCCCTACGGCAGATGTCCCACTG
  CGGCAGATATCAACACACCCGATGTACCCATCTGCGTGTACGACCCCCTGCCACTGATTCTGCTGGGCAT
  TCTTCTTGGCGTGGCAGTCATTATCATTGTTGTCCTTCTGCTGTATTTCATGGTGGACAATGGGACCCGG
  CTGCACGACGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100658 representing YP_003038521
  Red=Cloning sites Green=Tags
  
  MFLLPRFVLVSCIIGSLGFENPPTNVVSHLNGDWFLFGDSRSDCNHVVNTNPRNYSYMDLNPALCDSGKI
  SSKAGNSIFRSFHFTDFYNYTGEGQQIIFYEGVNFTPYHAFKCTTSGSNDIWMQNKGLFYTQVYKNMAVY
  RSLTFVNVPYVYNGSAQSTALCKSGSLVLNNPAYIAREANFGDYYYKVEADFYLSGCDEYIVPLCIFNGK
  FLSNTKYYDDSQYYFNKDTGVIYGLNSTETITTGFDFNCHYLVLPSGNYLAISNELLLTVPTKAICLNKR
  KDFTPVQVVDSRWNNARQSDNMTAVACQPPYCYFRNSTTNYVGVYDINHGDAGFTSILSGLLYDSPCFSQ
  QGVFRYNNVSSVWPLYPYGRCPTAADINTPDVPICVYDPLPLILLGILLGVAVIIIVVLLLYFMVDNGTR
  LHDA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012950
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012950.1, YP_003038521
• RefSeq ORF: 1272
• MW: 47.7 kDa