membrane protein (NC_012950) Virus Tagged ORF Clone

CAT#: VC100664

Myc-DDK-tagged ORF clone of viral ORF for membrane protein [Human enteric coronavirus strain 4408], codon optimized for human cell expression, YP_003038527

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: membrane protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100664 represents NCBI reference of YP_003038527 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCGTGACCACCCCCGCCCCCGTGTACACCTGGACCGCCGACGAAGCCATCAAGTTCCTGAAGG
  AATGGAACTTCTCCCTGGGGATCATCCTCCTGTTCATCACAGTGATCCTGCAATTCGGCTACACCAGCCG
  GAGCATGTTTGTGTACGTCATCAAGATGATCCTGTGGCTGATGTGGCCCCTGACTATCATCCTGACCATC
  TTCAACTGCGTGTACGCCCTGAACAACGTGTACCTGGGCTTCTCCATCGTCTTCACCATCGTCGCCATCA
  TCATGTGGATCGTGTACTTTGTGAACAGCATCCGGCTGTTCATCCGGACCGGCAGCTGGTGGAGCTTCAA
  TCCCGAAACCAACAACCTGATGTGCATCGATATGAAGGGCCGGATGTACGTGCGGCCCATCATTGAGGAC
  TACCACACCCTGACTGTCACTATCATCCGGGGCCACCTGTACATGCAGGGCATCAAGCTGGGCACCGGCT
  ATAGCCTCAGCGACCTCCCCGCCTATGTGACCGTCGCTAAAGTCAGCCACCTGCTGACCTACAAGCGGGG
  CTTTCTGGACAAGATCGGCGACACCAGCGGCTTCGCCGTGTACGTGAAGAGCAAGGTGGGCAACTACCGG
  CTGCCAAGCACTCAAAAGGGCAGCGGCATGGACACAGCCCTGCTGAGAAATAACATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100664 representing YP_003038527
  Red=Cloning sites Green=Tags
  
  MSSVTTPAPVYTWTADEAIKFLKEWNFSLGIILLFITVILQFGYTSRSMFVYVIKMILWLMWPLTIILTI
  FNCVYALNNVYLGFSIVFTIVAIIMWIVYFVNSIRLFIRTGSWWSFNPETNNLMCIDMKGRMYVRPIIED
  YHTLTVTIIRGHLYMQGIKLGTGYSLSDLPAYVTVAKVSHLLTYKRGFLDKIGDTSGFAVYVKSKVGNYR
  LPSTQKGSGMDTALLRNNI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012950
• ORF Size: 687 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012950.1, YP_003038527
• RefSeq ORF: 687
• MW: 26.4 kDa