1A(VP4) (NC_001472) Virus Tagged ORF Clone

CAT#: VC100728

Myc-DDK-tagged ORF clone of viral ORF for 1A(VP4) [Human enterovirus B], codon optimized for human cell expression, NP_740537

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 1A(VP4)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100728 represents NCBI reference of NP_740537 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGGCGCACAGGTTTCTACCCAAAAGACTGGCGCCCACGAAACAGGGCTCAACGCTTCAGGAAATT
  CTATTATACACTACACTAACATCAACTACTACAAAGATGCAGCCAGTAATTCTGCGAACAGGCAGGATTT
  TACTCAGGACCCCGGCAAGTTTACCGAGCCTGTCAAGGATATCATGATCAAATCCATGCCTGCTCTTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100728 representing NP_740537
  Red=Cloning sites Green=Tags
  
  MMGAQVSTQKTGAHETGLNASGNSIIHYTNINYYKDAASNSANRQDFTQDPGKFTEPVKDIMIKSMPALN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001472
• ORF Size: 210 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001472.1, NP_740537
• RefSeq ORF: 210
• MW: 7.6 kDa