1C(VP3) (NC_001472) Virus Tagged ORF Clone

CAT#: VC100730

Myc-DDK-tagged ORF clone of viral ORF for 1C(VP3) [Human enterovirus B], codon optimized for human cell expression, NP_740539

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 1C(VP3)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100730 represents NCBI reference of NP_740539 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCTCCCCGTAATGACAACCCCAGGTAGTACACAGTTTTTGACCAGTGATGACTTCCAATCCCCCA
  GTGCAATGCCACAGTTCGACGTGACCCCGGAAATGCAGATCCCTGGCAGGGTAAACAATCTGATGGAAAT
  TGCAGAAGTGGATTCAGTAGTTCCCGTGAACAATACCGATAACAACGTGAACGGACTTAAAGCTTATCAG
  ATCCCCGTGCAGAGTAATTCTGATAACCGGAGGCAGGTGTTCGGATTTCCTCTCCAGCCCGGAGCCAACA
  ACGTGCTGAACAGAACGCTGTTGGGTGAGATTCTCAACTATTACACTCACTGGTCAGGATCCATTAAACT
  CACATTTATGTTCTGTGGGTCCGCCATGGCTACCGGAAAGTTTCTGCTCGCATACAGTCCACCTGGAGCA
  GGCGTGCCAAAGAACAGAAGAGACGCTATGCTGGGCACACACGTAATCTGGGACGTCGGGCTGCAGTCCT
  CTTGTGTGCTGTGCGTTCCCTGGATCTCTCAGACACACTACCGCTATGTCGTTGAGGATGAATACACAGC
  TGCCGGCTACGTTACCTGCTGGTATCAAACGAACATAATTGTTCCCGCGGATGTTCAGTCCACGTGTGAC
  ATTCTTTGCTTCGTTTCCGCTTGCAACGATTTTTCTGTACGCATGCTGAAGGACACCCCCTTCATTCGCC
  AGGATAACTTCTACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100730 representing NP_740539
  Red=Cloning sites Green=Tags
  
  MGLPVMTTPGSTQFLTSDDFQSPSAMPQFDVTPEMQIPGRVNNLMEIAEVDSVVPVNNTDNNVNGLKAYQ
  IPVQSNSDNRRQVFGFPLQPGANNVLNRTLLGEILNYYTHWSGSIKLTFMFCGSAMATGKFLLAYSPPGA
  GVPKNRRDAMLGTHVIWDVGLQSSCVLCVPWISQTHYRYVVEDEYTAAGYVTCWYQTNIIVPADVQSTCD
  ILCFVSACNDFSVRMLKDTPFIRQDNFYQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001472
• ORF Size: 717 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001472.1, NP_740539
• RefSeq ORF: 717
• MW: 26.6 kDa