1D(VP1) (NC_001472) Virus Tagged ORF Clone

CAT#: VC100731

Myc-DDK-tagged ORF clone of viral ORF for 1D(VP1) [Human enterovirus B], codon optimized for human cell expression, NP_740540

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 1D(VP1)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100731 represents NCBI reference of NP_740540 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCGGTGGAGGAGTCAGTTGAGAGAGCTATGGTGAGAGTTGCTGATACCGTTTCCTCCAAGCCTA
  CGAATTCCGAATCCATCCCTGCGCTGACGGCTGCAGAGACCGGCCATACTTCTCAGGTGGTTCCGTCCGA
  TACGATGCAGACGCGCCATGTGAAGAACTATCATTCTCGCTCCGAATCCTCTATCGAGAACTTCCTGTGC
  AGGTCCGCATGTGTGTACTACGCCACCTACAATAACAACTCAGAAAAAGGGTATGCTGAATGGGTGATTA
  ATACAAGGCAAGTCGCACAGCTGCTGAGGCGGAAACTCGAGTTTACTTACCTCAGATTCGATCTCGAGCT
  CACTTTTGTGATCACCAGCGCACAGGAACCATCCACAGCTACCAGCGTCGACGCCCCAGTCCAAACCCAG
  CAGATTATGTACGTCCCTCCAGGCGGCCCCGTGCCCACAAAAGTCACCGATTACGCTTGGCAGACCTCTA
  CCAACCCAAGTGTTTTCTGGACAGAAGGAAACGCTCCCCCAAGGATGTCCATTCCCTTCATTAGCATAGG
  AAACGCATATTCCTGCTTCTACGATGGGTGGACACAGTTCAGCCGCAACGGAGTCTACGGCATAAATACA
  TTGAACAATATGGGTACTTTGTATATGCGCCATGTGAACGAAGCAGGTCAGGGGCCAATCAAATCTACCG
  TACGCATTTATTTTAAACCTAAACATGTAAAGGCTTGGGTGCCTCGACCGCCAAGACTTTGCCAGTATGA
  AAAGCAGAAGAACGTTAACTTTAACCCCACCGGGGTGACTACTACTAGAAGCAACATTACTACGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100731 representing NP_740540
  Red=Cloning sites Green=Tags
  
  MGPVEESVERAMVRVADTVSSKPTNSESIPALTAAETGHTSQVVPSDTMQTRHVKNYHSRSESSIENFLC
  RSACVYYATYNNNSEKGYAEWVINTRQVAQLLRRKLEFTYLRFDLELTFVITSAQEPSTATSVDAPVQTQ
  QIMYVPPGGPVPTKVTDYAWQTSTNPSVFWTEGNAPPRMSIPFISIGNAYSCFYDGWTQFSRNGVYGINT
  LNNMGTLYMRHVNEAGQGPIKSTVRIYFKPKHVKAWVPRPPRLCQYEKQKNVNFNPTGVTTTRSNITTT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001472
• ORF Size: 837 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001472.1, NP_740540
• RefSeq ORF: 837
• MW: 31.3 kDa