2A (NC_001472) Virus Tagged ORF Clone

CAT#: VC100732

Myc-DDK-tagged ORF clone of viral ORF for 2A [Human enterovirus B], codon optimized for human cell expression, NP_740541

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100732 represents NCBI reference of NP_740541 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCTTTTGGGCAGCAGTCTGGAGCAGTCTATGTGGGGAACTACAGGGTTGTGAACAGACACCTCG
  CTACACGCGAAGACTGGCAGAGGTGCGTGTGGGAGGACTATAACCGGGATCTGCTGGTGAGTACCACAAC
  AGCCCACGGTTGCGATATTATTGCCCGCTGCCAGTGTACTACGGGCGTCTATTTCTGCGCTTCTCGGAAC
  AAACACTACCCCGTAAGCTTTGAAGGTCCAGGCCTGGTCGAGGTTCAAGAGTCAGAATATTACCCTAAGA
  GGTACCAGTCCCACGTTCTGCTCGCCGCTGGATTCAGCGAACCTGGAGACTGTGGAGGTATTCTGCGGTG
  CGAACATGGCGTAGTTGGCATAGTAACGATGGGCGGGGAAGGCGTGGTGGGCTTTGCTGATGTAAGAGAC
  CTGCTGTGGCTTGAAGATGATGCTATGGAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100732 representing NP_740541
  Red=Cloning sites Green=Tags
  
  MGAFGQQSGAVYVGNYRVVNRHLATREDWQRCVWEDYNRDLLVSTTTAHGCDIIARCQCTTGVYFCASRN
  KHYPVSFEGPGLVEVQESEYYPKRYQSHVLLAAGFSEPGDCGGILRCEHGVVGIVTMGGEGVVGFADVRD
  LLWLEDDAMEQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001472
• ORF Size: 453 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001472.1, NP_740541
• RefSeq ORF: 453
• MW: 16.8 kDa