1B(VP2) (NC_001472) Virus Tagged ORF Clone

CAT#: VC100729

Myc-DDK-tagged ORF clone of viral ORF for 1B(VP2) [Human enterovirus B], codon optimized for human cell expression, NP_740538

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 1B(VP2)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100729 represents NCBI reference of NP_740538 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCCTTCAGCAGAAGAATGTGGTTACTCCGACCGAGTTCGCAGTATAACATTGGGAAATAGCACGA
  TAACCACCCAGGAATGTGCCAACGTGGTGGTTGGGTACGGGGTGTGGCCTGAATACCTGAAGGATAACGA
  GGCTACTGGAGAAGACCAGCCAACCCAACCGGATGTGGCCACCTGTAGATTCTATACACTGGAGAGTGTG
  CAGTGGATGAAAAATTCCGCCGGCTGGTGGTGGAAACTGCCTGACGCCCTCAGCCAGATGGGATTGTTTG
  GCCAGAACATGCAATACCACTACCTGGGGCGCACAGGGTACACGATTCATGTGCAGTGCAATGCATCAAA
  GTTTCATCAGGGCTGCCTGCTGGTGGTGTGCGTGCCCGAAGCTGAAATGGGATGTAGCAACTTGAACAAC
  ACGCCGAAGTTTGCCGAACTGTCAGGCGGAGATAACGCACGCATGTTCACCGATACCGAAGTAGGCACCT
  CTAATGATAAAAAAGTGCAGACAGCCGTGTGGAACGCTGGGATGGGGGTCGGCGTGGGGAATTTGACCAT
  CTTCCCCCATCAGTGGATCAATTTGCGGACCAATAATAGTGCCACTATTGTCATGCCATACATCAACTCC
  GTGCCAATGGACAATATGTATCGCCACAATAATTTGACTCTCATGATCATCCCATTTGTACCCTTGAACT
  ACAGCGAAGGGTCATCACCCTACGTACCCATAACTGTGACAATCGCCCCAATGTGCGCAGAATACAACGG
  CCTCAGACTTGCCTCTTCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100729 representing NP_740538
  Red=Cloning sites Green=Tags
  
  MSPSAEECGYSDRVRSITLGNSTITTQECANVVVGYGVWPEYLKDNEATGEDQPTQPDVATCRFYTLESV
  QWMKNSAGWWWKLPDALSQMGLFGQNMQYHYLGRTGYTIHVQCNASKFHQGCLLVVCVPEAEMGCSNLNN
  TPKFAELSGGDNARMFTDTEVGTSNDKKVQTAVWNAGMGVGVGNLTIFPHQWINLRTNNSATIVMPYINS
  VPMDNMYRHNNLTLMIIPFVPLNYSEGSSPYVPITVTIAPMCAEYNGLRLASSQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001472
• ORF Size: 792 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001472.1, NP_740538
• RefSeq ORF: 792
• MW: 29.2 kDa