1B(VP2) (NC_001472) Virus Tagged ORF Clone

CAT#: VC100729

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for 1B(VP2) [Human enterovirus B], codon optimized for human cell expression, NP_740538


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Reconstitution Protocol

USD 400.00

3 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol 1B(VP2)
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100729 represents NCBI reference of NP_740538 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTCCTTCAGCAGAAGAATGTGGTTACTCCGACCGAGTTCGCAGTATAACATTGGGAAATAGCACGA
TAACCACCCAGGAATGTGCCAACGTGGTGGTTGGGTACGGGGTGTGGCCTGAATACCTGAAGGATAACGA
GGCTACTGGAGAAGACCAGCCAACCCAACCGGATGTGGCCACCTGTAGATTCTATACACTGGAGAGTGTG
CAGTGGATGAAAAATTCCGCCGGCTGGTGGTGGAAACTGCCTGACGCCCTCAGCCAGATGGGATTGTTTG
GCCAGAACATGCAATACCACTACCTGGGGCGCACAGGGTACACGATTCATGTGCAGTGCAATGCATCAAA
GTTTCATCAGGGCTGCCTGCTGGTGGTGTGCGTGCCCGAAGCTGAAATGGGATGTAGCAACTTGAACAAC
ACGCCGAAGTTTGCCGAACTGTCAGGCGGAGATAACGCACGCATGTTCACCGATACCGAAGTAGGCACCT
CTAATGATAAAAAAGTGCAGACAGCCGTGTGGAACGCTGGGATGGGGGTCGGCGTGGGGAATTTGACCAT
CTTCCCCCATCAGTGGATCAATTTGCGGACCAATAATAGTGCCACTATTGTCATGCCATACATCAACTCC
GTGCCAATGGACAATATGTATCGCCACAATAATTTGACTCTCATGATCATCCCATTTGTACCCTTGAACT
ACAGCGAAGGGTCATCACCCTACGTACCCATAACTGTGACAATCGCCCCAATGTGCGCAGAATACAACGG
CCTCAGACTTGCCTCTTCACAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100729 representing NP_740538
Red=Cloning sites Green=Tags

MSPSAEECGYSDRVRSITLGNSTITTQECANVVVGYGVWPEYLKDNEATGEDQPTQPDVATCRFYTLESV
QWMKNSAGWWWKLPDALSQMGLFGQNMQYHYLGRTGYTIHVQCNASKFHQGCLLVVCVPEAEMGCSNLNN
TPKFAELSGGDNARMFTDTEVGTSNDKKVQTAVWNAGMGVGVGNLTIFPHQWINLRTNNSATIVMPYINS
VPMDNMYRHNNLTLMIIPFVPLNYSEGSSPYVPITVTIAPMCAEYNGLRLASSQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001472
ORF Size 792 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_001472.1, NP_740538
RefSeq ORF 792
MW 29.2 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.