2C (NC_001472) Virus Tagged ORF Clone

CAT#: VC100734

Myc-DDK-tagged ORF clone of viral ORF for 2C [Human enterovirus B], codon optimized for human cell expression, NP_740543

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100734 represents NCBI reference of NP_740543 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCCGGATGGCTGAAAAAGTTTACGGAGATGACAAATGCATGTAAGGGGATGGAATGGATTGCAA
  TAAAAATTCAGAAATTTATCGAATGGCTCAAAGTGAAGATCCTCCCAGAGGTGAAAGAAAAGCACGAATT
  CCTCAACAGACTGAAGCAGCTGCCACTGCTGGAAAGCCAGATCGCAACTATAGAACAGAGCGCACCTTCC
  CAGTCTGACCAGGAGCAGCTTTTTTCTAATGTGCAGTATTTCGCACACTACTGCCGGAAGTACGCCCCAC
  TGTACGCCGCCGAGGCCAAGAGGGTCTTTTCTTTGGAAAAAAAGATGAGCAACTATATCCAGTTCAAATC
  AAAGTGCAGGATCGAGCCCGTCTGTCTGCTTCTCCACGGCTCCCCTGGGGCTGGCAAGAGCGTCGCAACG
  AATCTGATCGGCAGGAGTCTGGCCGAGAAACTCAATTCTAGCGTTTATTCACTGCCTCCTGATCCTGATC
  ATTTTGACGGGTATAAGCAACAGGCTGTGGTTATTATGGACGACCTCTGTCAAAATCCTGACGGTAAGGA
  TGTCAGCCTCTTCTGTCAGATGGTATCCTCTGTCGACTTCGTCCCCCCCATGGCCGCTCTCGAAGAAAAG
  GGGATCCTGTTCACCAGCCCTTTCGTCCTGGCGTCTACCAACGCTGGTTCAATCAACGCTCCAACGGTCT
  CCGACTCTCGGGCGCTGGCCCGGAGGTTTCACTTTGATATGAACATCGAGGTGATATCTATGTACTCACA
  GAATGGGAAGATAAACATGCCAATGTCCGTTAAGACTTGTGACGAAGAGTGCTGTCCTGTGAATTTTAAG
  AAGTGTTGTCCATTGGTCTGCGGGAAAGCCATCCAGTTCATCGACCGAAGAACTCAAGTACGATATTCCC
  TGGATATGCTTGTGACCGAGATGTTCAGAGAGTACAATCATCGGCACTCTGTCGGAGCTACTCTCGAAGC
  ACTTTTTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100734 representing NP_740543
  Red=Cloning sites Green=Tags
  
  MNSGWLKKFTEMTNACKGMEWIAIKIQKFIEWLKVKILPEVKEKHEFLNRLKQLPLLESQIATIEQSAPS
  QSDQEQLFSNVQYFAHYCRKYAPLYAAEAKRVFSLEKKMSNYIQFKSKCRIEPVCLLLHGSPGAGKSVAT
  NLIGRSLAEKLNSSVYSLPPDPDHFDGYKQQAVVIMDDLCQNPDGKDVSLFCQMVSSVDFVPPMAALEEK
  GILFTSPFVLASTNAGSINAPTVSDSRALARRFHFDMNIEVISMYSQNGKINMPMSVKTCDEECCPVNFK
  KCCPLVCGKAIQFIDRRTQVRYSLDMLVTEMFREYNHRHSVGATLEALFQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001472
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001472.1, NP_740543
• RefSeq ORF: 990
• MW: 37.4 kDa