E6 (NC_001357) Virus Tagged ORF Clone

CAT#: VC101803

Myc-DDK-tagged ORF clone of viral ORF for E6 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040310

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101803 represents NCBI reference of NP_040310 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGCTTTGAAGATCCCACGCGGCGGCCCTACAAGCTGCCTGATCTGTGCACCGAGCTGAACACTA
  GTCTGCAGGACATCGAAATAACATGCGTCTACTGCAAAACTGTGCTCGAACTCACAGAAGTGTTCGAATT
  TGCGTTTAAGGACCTTTTCGTGGTTTATAGAGACTCAATTCCACACGCCGCCTGTCACAAGTGTATCGAT
  TTCTACAGCAGGATCCGCGAACTGCGACACTACTCCGATTCTGTCTATGGGGATACTCTTGAAAAGCTGA
  CGAATACCGGTCTGTACAACCTGCTGATACGCTGCCTTAGGTGCCAGAAGCCGTTGAACCCAGCTGAAAA
  GCTCAGACACCTCAACGAGAAAAGAAGATTCCATAATATTGCAGGCCACTATAGGGGACAGTGCCACAGT
  TGCTGTAATAGAGCACGACAAGAGCGGCTGCAAAGGCGAAGGGAAACTCAAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101803 representing NP_040310
  Red=Cloning sites Green=Tags
  
  MARFEDPTRRPYKLPDLCTELNTSLQDIEITCVYCKTVLELTEVFEFAFKDLFVVYRDSIPHAACHKCID
  FYSRIRELRHYSDSVYGDTLEKLTNTGLYNLLIRCLRCQKPLNPAEKLRHLNEKRRFHNIAGHYRGQCHS
  CCNRARQERLQRRRETQV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001357
• ORF Size: 474 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001357.1, NP_040310
• RefSeq ORF: 474
• Locus ID: 1489088
• MW: 18.9 kDa