L2 (NC_001357) Virus Tagged ORF Clone

CAT#: VC101809

Myc-DDK-tagged ORF clone of viral ORF for L2 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040316

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101809 represents NCBI reference of NP_040316 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTCTCATAGAGCCGCTCGGAGGAAGAGAGCGTCCGTGACAGATTTGTATAAGACCTGCAAGCAGA
  GCGGAACTTGCCCTCCCGACGTCGTCCCTAAGGTCGAGGGGACCACCCTGGCAGATAAGATCTTGCAGTG
  GAGCTCCCTGGGGATCTTCCTGGGAGGGCTCGGTATTGGGACCGGGAGTGGCACTGGAGGCAGAACAGGC
  TATATTCCTCTGGGAGGAAGATCCAACACAGTGGTGGACGTCGGTCCAACTAGACCCCCAGTGGTGATCG
  AACCTGTTGGACCGACCGACCCATCCATCGTAACACTGATTGAGGACAGTTCCGTGGTCACCTCTGGCGC
  TCCAAGGCCCACCTTTACCGGTACCAGCGGATTCGATATTACAAGTGCTGGCACCACGACCCCCGCCGTC
  CTCGATATCACACCCTCTAGCACAAGCGTAAGCATCTCCACAACTAACTTTACTAACCCCGCATTTAGCG
  ATCCTAGCATCATTGAGGTTCCCCAGACCGGTGAGGTTGCTGGCAACGTGTTCGTGGGCACACCCACCTC
  AGGAACCCACGGGTACGAAGAGATTCCACTCCAGACCTTTGCGAGTAGTGGGACAGGGGAAGAGCCAATC
  TCCAGTACCCCACTGCCGACGGTGCGGAGGGTGGCAGGCCCGCGGCTGTACTCTAGGGCCTACCAGCAGG
  TAAGCGTAGCCAACCCAGAATTCCTTACAAGGCCCTCTTCCCTGATCACATACGACAACCCAGCCTTCGA
  ACCTGTGGACACTACTTTGACCTTCGACCCAAGATCCGACGTGCCCGACTCCGATTTTATGGATATCATC
  AGGTTGCATCGCCCTGCCCTGACATCACGGCGGGGGACCGTGAGATTTAGCCGGCTTGGCCAGCGCGCCA
  CAATGTTTACCCGATCCGGCACCCAGATTGGAGCACGGGTGCATTTCTACCACGACATCTCTCCTATCGC
  ACCCAGCCCTGAGTACATTGAGCTGCAGCCGCTGGTGAGCGCTACCGAAGACAATGACTTGTTTGACATC
  TACGCTGACGACATGGATCCGGCAGTTCCCGTCCCTAGCCGCAGCACCACGTCTTTCGCCTTTTTTAAGT
  ATTCTCCAACCATAAGTAGTGCCAGCAGCTATAGTAACGTGACTGTCCCGTTGACCAGCAGCTGGGACGT
  TCCCGTCTATACCGGCCCCGACATTACACTGCCTTCCACCACGTCTGTATGGCCCATAGTTTCCCCAACT
  GCCCCAGCCTCCACACAGTACATTGGTATTCACGGCACCCACTACTATCTCTGGCCGCTGTACTACTTCA
  TTCCCAAAAAACGAAAGAGGGTGCCTTATTTCTTCGCAGACGGATTTGTGGCCGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101809 representing NP_040316
  Red=Cloning sites Green=Tags
  
  MVSHRAARRKRASVTDLYKTCKQSGTCPPDVVPKVEGTTLADKILQWSSLGIFLGGLGIGTGSGTGGRTG
  YIPLGGRSNTVVDVGPTRPPVVIEPVGPTDPSIVTLIEDSSVVTSGAPRPTFTGTSGFDITSAGTTTPAV
  LDITPSSTSVSISTTNFTNPAFSDPSIIEVPQTGEVAGNVFVGTPTSGTHGYEEIPLQTFASSGTGEEPI
  SSTPLPTVRRVAGPRLYSRAYQQVSVANPEFLTRPSSLITYDNPAFEPVDTTLTFDPRSDVPDSDFMDII
  RLHRPALTSRRGTVRFSRLGQRATMFTRSGTQIGARVHFYHDISPIAPSPEYIELQPLVSATEDNDLFDI
  YADDMDPAVPVPSRSTTSFAFFKYSPTISSASSYSNVTVPLTSSWDVPVYTGPDITLPSTTSVWPIVSPT
  APASTQYIGIHGTHYYLWPLYYFIPKKRKRVPYFFADGFVAA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001357
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001357.1, NP_040316
• RefSeq ORF: 1386
• Locus ID: 1489091
• MW: 49.6 kDa