E4 (NC_001357) Virus Tagged ORF Clone

CAT#: VC101807

Myc-DDK-tagged ORF clone of viral ORF for E4 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040314

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101807 represents NCBI reference of NP_040314 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTCTGCGCTGTCCCGGTGACAACACGGTACCCCCTCCTGTCTCTGCTGAACTCATACTCAACGC
  CTCCGCATAGAATTCCTGCGCCATGTCCTTGGGCTCCCCAGCGGCCTACCGCACGGAGAAGACTGCTGCA
  CGATCTTGACACCGTTGACAGCAGGAGGAGCTCTATTGTCGATTTGTCCACCCACTTTTCTGTACAGCTG
  CACCTCCAAGCAACAACCAAGGATGGTAACTCCGTCGTAGTGACACTGAGGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101807 representing NP_040314
  Red=Cloning sites Green=Tags
  
  MTLCAVPVTTRYPLLSLLNSYSTPPHRIPAPCPWAPQRPTARRRLLHDLDTVDSRRSSIVDLSTHFSVQL
  HLQATTKDGNSVVVTLRL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001357
• ORF Size: 264 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001357.1, NP_040314
• RefSeq ORF: 264
• Locus ID: 1489086
• MW: 9.9 kDa