E2 (NC_001357) Virus Tagged ORF Clone

CAT#: VC101806

Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040313

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101806 represents NCBI reference of NP_040313 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGACGCCGAAGGAGACCCTGTCCGAGCGCCTGTCATGTGTGCAAGACAAGATCATCGACCATTATG
  AAAATGACAGCAAAGATATTGACAGCCAAATTCAATACTGGCAGCTTATCAGGTGGGAAAACGCTATCTT
  TTTTGCCGCCCGGGAACACGGCATTCAAACTCTGAACCACCAGGTTGTTCCTGCTTATAATATCTCCAAG
  TCCAAAGCTCATAAGGCAATAGAGCTGCAGATGGCACTGCAAGGACTGGCTCAGAGCAGATATAAAACCG
  AAGACTGGACACTGCAGGACACTTGTGAGGAGTTGTGGAATACTGAACCCACCCACTGCTTCAAAAAAGG
  CGGCCAAACTGTGCAGGTTTACTTCGACGGAAATAAGGACAACTGCATGACATATGTGGCATGGGATAGC
  GTCTACTATATGACAGACGCAGGAACATGGGATAAGACAGCAACCTGCGTCAGCCATCGCGGCCTGTATT
  ATGTGAAGGAGGGTTATAACACCTTTTACATCGAATTCAAGTCTGAATGTGAAAAATATGGCAACACCGG
  CACTTGGGAGGTGCACTTTGGAAATAACGTCATCGATTGCAATGACTCAATGTGTAGCACCAGTGACGAT
  ACAGTGTCTGCCACGCAGCTGGTCAAGCAGCTCCAGCACACCCCGAGCCCTTATAGTAGCACTGTGAGCG
  TAGGCACAGCGAAAACCTACGGGCAAACGTCCGCCGCAACCAGGCCAGGCCACTGCGGGCTGGCTGAAAA
  GCAGCACTGCGGACCAGTGAATCCTCTGTTGGGCGCCGCCACCCCCACTGGAAACAATAAGCGGAGAAAG
  CTGTGCTCCGGCAACACCACCCCCATCATCCATCTCAAAGGCGACAGAAACAGTCTTAAGTGCCTCCGGT
  ATCGCCTTCGAAAACACAGCGATCATTACAGAGATATTAGTTCAACTTGGCATTGGACCGGTGCGGGTAA
  CGAAAAGACAGGCATCCTGACTGTGACGTATCATTCCGAAACTCAAAGAACAAAGTTCCTGAATACCGTC
  GCTATTCCAGACAGCGTTCAGATTCTCGTTGGCTACATGACCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101806 representing NP_040313
  Red=Cloning sites Green=Tags
  
  MQTPKETLSERLSCVQDKIIDHYENDSKDIDSQIQYWQLIRWENAIFFAAREHGIQTLNHQVVPAYNISK
  SKAHKAIELQMALQGLAQSRYKTEDWTLQDTCEELWNTEPTHCFKKGGQTVQVYFDGNKDNCMTYVAWDS
  VYYMTDAGTWDKTATCVSHRGLYYVKEGYNTFYIEFKSECEKYGNTGTWEVHFGNNVIDCNDSMCSTSDD
  TVSATQLVKQLQHTPSPYSSTVSVGTAKTYGQTSAATRPGHCGLAEKQHCGPVNPLLGAATPTGNNKRRK
  LCSGNTTPIIHLKGDRNSLKCLRYRLRKHSDHYRDISSTWHWTGAGNEKTGILTVTYHSETQRTKFLNTV
  AIPDSVQILVGYMTM
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001357
• ORF Size: 1095 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001357.1, NP_040313
• RefSeq ORF: 1095
• Locus ID: 1489085
• MW: 41.4 kDa