E1 (NC_001357) Virus Tagged ORF Clone

CAT#: VC101805

Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040312

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101805 represents NCBI reference of NP_040312 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATCCCGAGGGCACTGATGGCGAGGGCACAGGATGTAATGGTTGGTTCTATGTGCAAGCCATCG
  TGGATAAAAAAACTGGAGATGTTATTTCTGACGACGAAGATGAGAATGCCACCGACACTGGCAGTGATAT
  GGTCGATTTTATAGACACCCAGGGGACATTCTGTGAACAAGCTGAGCTGGAGACTGCACAGGCTCTGTTC
  CATGCCCAGGAGGTGCACAATGATGCCCAAGTACTGCACGTCCTTAAAAGAAAGTTCGCGGGGGGCAGCA
  CGGAAAATAGTCCCCTCGGCGAGAGACTGGAAGTCGACACCGAACTCTCCCCCAGGCTCCAGGAGATCTC
  CCTTAATTCTGGACAGAAGAAGGCAAAACGGCGACTGTTCACAATTTCCGACTCCGGCTATGGATGTAGC
  GAGGTCGAGGCTACCCAGATCCAGGTTACAACCAATGGAGAGCACGGCGGAAACGTGTGCTCCGGAGGGT
  CTACCGAGGCCATAGATAACGGCGGGACAGAGGGCAACAATTCCTCCGTCGACGGCACCTCAGACAACTC
  AAACATCGAGAACGTGAATCCACAGTGCACGATTGCTCAACTGAAAGACCTGCTGAAGGTTAATAACAAG
  CAGGGCGCTATGCTCGCAGTGTTTAAGGACACCTATGGACTTAGCTTTACTGATCTGGTTCGAAATTTCA
  AATCCGACAAGACCACTTGCACAGACTGGGTTACGGCTATTTTTGGCGTTAACCCGACAATTGCCGAAGG
  GTTCAAAACTCTTATCCAGCCGTTCATTCTGTACGCACACATACAATGCCTCGATTGCAAGTGGGGAGTG
  CTGATCCTGGCACTTCTGAGGTACAAGTGTGGAAAGTCTAGGCTGACTGTAGCTAAAGGCTTGAGCACAC
  TGTTGCACGTGCCAGAGACATGCATGCTGATCCAGCCACCTAAACTCCGGAGCTCCGTCGCCGCTCTGTA
  TTGGTACCGCACTGGAATCAGTAACATCTCTGAGGTGATGGGAGACACCCCGGAGTGGATTCAGAGACTC
  ACAATCATACAGCACGGCATTGACGACTCTAATTTTGACTTGAGCGAAATGGTGCAGTGGGCCTTTGACA
  ATGAACTGACCGATGAATCCGATATGGCCTTTGAATATGCACTGCTCGCAGATAGTAATTCTAACGCCGC
  CGCATTCCTGAAATCTAACTGTCAGGCCAAGTATCTCAAAGACTGTGCCACTATGTGCAAGCATTACCGG
  CGCGCCCAAAAGCGGCAGATGAACATGTCACAGTGGATCAGGTTCCGGTGTTCCAAGATAGACGAAGGAG
  GCGATTGGAGGCCGATCGTGCAGTTCCTCCGGTACCAACAAATCGAGTTTATAACGTTTTTGGGTGCCTT
  GAAGTCATTTCTCAAAGGCACCCCAAAGAAGAACTGTCTGGTTTTCTGCGGACCGGCGAACACCGGCAAA
  TCTTACTTCGGCATGTCATTCATCCATTTCATTCAAGGAGCTGTTATTAGTTTTGTGAACTCCACGTCCC
  ACTTCTGGCTCGAGCCTCTGACCGACACTAAAGTCGCTATGCTGGACGACGCGACAACCACATGCTGGAC
  TTATTTTGACACATACATGCGGAATGCCCTCGATGGCAACCCCATAAGCATCGATAGAAAGCACAAGCCT
  TTGATTCAGCTTAAGTGTCCCCCCATCCTGCTGACAACCAACATTCACCCAGCTAAGGATAACAGGTGGC
  CGTATCTGGAGAGCCGCATCACAGTATTTGAGTTTCCCAACGCTTTCCCTTTTGACAAGAACGGAAACCC
  TGTTTACGAGATCAACGACAAAAACTGGAAATGCTTCTTCGAGCGCACCTGGTCTCGATTGGATTTGCAC
  GAGGAGGAGGAAGACGCTGACACTGAGGGCAACCCATTCGGCACCTTCAAGCTGCGGGCTGGCCAGAACC
  ATCGCCCGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101805 representing NP_040312
  Red=Cloning sites Green=Tags
  
  MADPEGTDGEGTGCNGWFYVQAIVDKKTGDVISDDEDENATDTGSDMVDFIDTQGTFCEQAELETAQALF
  HAQEVHNDAQVLHVLKRKFAGGSTENSPLGERLEVDTELSPRLQEISLNSGQKKAKRRLFTISDSGYGCS
  EVEATQIQVTTNGEHGGNVCSGGSTEAIDNGGTEGNNSSVDGTSDNSNIENVNPQCTIAQLKDLLKVNNK
  QGAMLAVFKDTYGLSFTDLVRNFKSDKTTCTDWVTAIFGVNPTIAEGFKTLIQPFILYAHIQCLDCKWGV
  LILALLRYKCGKSRLTVAKGLSTLLHVPETCMLIQPPKLRSSVAALYWYRTGISNISEVMGDTPEWIQRL
  TIIQHGIDDSNFDLSEMVQWAFDNELTDESDMAFEYALLADSNSNAAAFLKSNCQAKYLKDCATMCKHYR
  RAQKRQMNMSQWIRFRCSKIDEGGDWRPIVQFLRYQQIEFITFLGALKSFLKGTPKKNCLVFCGPANTGK
  SYFGMSFIHFIQGAVISFVNSTSHFWLEPLTDTKVAMLDDATTTCWTYFDTYMRNALDGNPISIDRKHKP
  LIQLKCPPILLTTNIHPAKDNRWPYLESRITVFEFPNAFPFDKNGNPVYEINDKNWKCFFERTWSRLDLH
  EEEEDADTEGNPFGTFKLRAGQNHRPL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001357
• ORF Size: 1971 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001357.1, NP_040312
• RefSeq ORF: 1971
• Locus ID: 1489084
• MW: 73.7 kDa