E7 (NC_001357) Virus Tagged ORF Clone

CAT#: VC101804

Myc-DDK-tagged ORF clone of viral ORF for E7 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040311

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101804 represents NCBI reference of NP_040311 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGGTCCAAAAGCTACCTTGCAGGACATTGTGTTGCATCTGGAACCGCAAAATGAGATCCCCGTCG
  ACCTCCTGTGCCATGAACAACTCTCCGACAGCGAGGAGGAAAATGACGAGATTGATGGCGTTAACCACCA
  GCATTTGCCAGCTAGGCGCGCTGAACCTCAACGCCACACAATGTTGTGTATGTGTTGCAAATGCGAAGCT
  AGGATCGAGCTGGTCGTGGAATCTTCCGCCGATGACCTCCGCGCCTTCCAACAGCTGTTCCTGAACACAC
  TCTCTTTCGTCTGCCCCTGGTGTGCGAGTCAGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101804 representing NP_040311
  Red=Cloning sites Green=Tags
  
  MHGPKATLQDIVLHLEPQNEIPVDLLCHEQLSDSEEENDEIDGVNHQHLPARRAEPQRHTMLCMCCKCEA
  RIELVVESSADDLRAFQQLFLNTLSFVCPWCASQQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001357
• ORF Size: 315 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001357.1, NP_040311
• RefSeq ORF: 315
• Locus ID: 1489089
• MW: 12.0 kDa