LIF (NM_002309) Human Recombinant Protein

CAT#: TP723889

Purified recombinant protein of Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF)

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human LIF, the region of Ser23-Phe202, from gene Accession# NM_002309
• Tag: Tag Free
• Predicted MW: 20 kDa
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Purity: >97%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 1 x PBS, pH7.4
• Bioactivity: The ED50 is 0.03-0.12 ng/ml, corresponding to a specific activity of 8.3-33.3 x 106 units/mg.
• Endotoxin: Less than 1.0 EU per µg of protein as determine by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_002300
• Locus ID: 3976
• UniProt ID: P15018
• Cytogenetics: 22q12.2
• Refseq Size: 3987
• Refseq ORF: 606
• Synonyms: CDF; DIA; HILDA; MLPLI
• Summary: 'The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]'
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway