Secondary Antibodies

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Rabbit IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in rabbit serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
Reactivities Rabbit
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled Rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rabbit IgA (Fc specific) goat polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in rabbit serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/100 - 1/1000.
Reactivities Rabbit
Conjugation Biotin
Immunogen Purified polyclonal IgA isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rabbit IgA (Fc specific) goat polyclonal antibody, FITC

Applications In immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80.
Reactivities Rabbit
Conjugation FITC
Immunogen Pools of purified IgA isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rabbit IgA (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in rabbit serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000.
Reactivities Rabbit
Conjugation HRP
Immunogen Purified normal IgA isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Azide Free

Applications The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Reactivities Rabbit
Immunogen Highly purified IgG and pools of homogenous IgA and IgM isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rat IgG2b (subclass specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in Rat serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
Reactivities Rat
Conjugation HRP
Immunogen Pools of purified homogenous IgG2b isolated from Rat sera.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rat IgG2ab (subclass specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000 - 1/10,000.
Reactivities Rat
Conjugation HRP
Immunogen Pools of purified homogenous IgG2a and IgG2b isolated from rat sera. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgG3 (subclass specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1.000 - 1/5,000.
Reactivities Mouse
Conjugation Biotin
Immunogen Pools of purified homogenous IgG3 isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgG3 (subclass specific) goat polyclonal antibody, FITC

Applications This antibody can be used to identify and measure IgG3, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions: 1/20-1/80.
Reactivities Mouse
Conjugation FITC
Immunogen Pools of purified homogenous IgG3 isolated from pooled Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgG3 (subclass specific) goat polyclonal antibody, HRP

Applications This antibody can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates To demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in Mouse serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical/Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/7,000 
depending on the method used. Working dilutions schould be stored at +4°C, not refrozen, and preferably used the same day.
Reactivities Mouse
Conjugation HRP
Immunogen Pools of purified homogenous IgG3 isolated from pooled Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/2000 and 1/10000.
Reactivities Monkey
Conjugation Biotin
Immunogen Highly purified IgG and pools of homogenous IgA and IgM isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/2000 and 1/10000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA + IgG + IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in monkey serum or other body fluids. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/2000 and 1/10000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified normal IgG , IgA and IgM isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA + IgG + IgM (Fc specific) goat polyclonal antibody, FITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/120.
Reactivities Monkey
Conjugation FITC
Immunogen Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA + IgG + IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/8000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/4000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified polyclonal IgA isolated from pooled Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80, depending on the method used.
Reactivities Monkey
Conjugation FITC
Immunogen Pools of purified IgA isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in monkey serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/10000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified normal IgA isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgG (Fc specific) goat polyclonal antibody, Azide Free

Applications As Unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry.
When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended Working Dilutions:
Histochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. 
Antibody Titre: Precipitin titre not less than 1/32 when tested against pooled normal Rhesus Monkey serum in agar-block immunodiffusion titration.
Reactivities Monkey
Immunogen Purified normal IgG isolated from pooled Rhesus Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgG (Fc specific) goat polyclonal antibody, Biotin

Applications This antibody can be used in Immunocytochemical and Immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates.
To demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases, To identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG in Monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions
Histochemical and Cytochemical Use:  1/100-1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/20000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled Rhesus Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgG (H+L chain) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgG (H+L chain) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of antigens or antibodies at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA, Western blotting). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/2000 and 1/10000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled Rhesus Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and cytochemical use are usually between 1/100 and 1/250.
ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/3000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgM (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working dilutions are usually between 1/20 and 1/80, depending on the method used.
Reactivities Monkey
Conjugation FITC
Immunogen Purified IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgM (Fc specific) goat polyclonal antibody, HRP

Applications ELISA. 
Dot blot. 
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
In Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of Monkey origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in Monkey serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled rhesus monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA (Secretory component) goat polyclonal antibody, Biotin

Applications Tested in Immunoelectrophoresis, Double Radial Immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes.
Can be used in Immunocytochemical and Immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in Monkey body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/200. 
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/1,000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified free monkey secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA (Secretory component) goat polyclonal antibody, FITC

Applications Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes.
Can be used as reagent for the direct detection of secretory component in monkey cells, tissues and body fluids in immunofluorescence; as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA).
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80.
Reactivities Chimpanzee, Monkey
Conjugation FITC
Immunogen Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Purified free monkey secretory component isolated from pooled milk is used for immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA (Secretory component) goat polyclonal antibody, HRP

Applications Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes.
In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in monkey milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working dilutions:
Histochemical and cytochemical 1/150 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays 1/200 -1/4000.
Reactivities Chimpanzee, Monkey
Conjugation HRP
Immunogen Secretory component can be present in monkey secretions bound to secretory IgA (sIgA) and in free form. Free monkey secretory component isolated from pooled milk is used for immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA (Secretory component) goat polyclonal antibody, TRITC

Applications Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes.
Can be used as reagent for the direct detection of secretory component in monkey cells, tissues and body fluids in immunofluorescence; as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA).
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:10 and 1:40.
Reactivities Chimpanzee, Monkey
Conjugation TRITC
Immunogen Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Purified free monkey secretory component isolated from pooled milk is used for immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Canine IgA (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in dog serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000.
Reactivities Canine
Conjugation HRP
Immunogen Purified normal IgA isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Canine IgG (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in Dog serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2000-1/10000.
Reactivities Canine
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled Dog serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Canine IgG (H+L chain) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of dog origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/10000.
Reactivities Canine
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Canine IgG (H+L chain) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in dog serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/10000.
Reactivities Canine
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Canine IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in dog serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/2000.
Reactivities Canine
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/400.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/8000 depending on the method used.
Reactivities Mouse
Conjugation HRP
Immunogen Purified homogenous IgM isolated from pooled mouse serum. Immunization with intact (19S) and split IgM (7S). Freund’s complete adjuvant is used in the first step of the immunization procedure.

Biotin Conjugated Goat Anti-Mouse IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Mouse IgG
Conjugation Biotin
Immunogen Mouse IgG, whole molecule

Human IgE goat polyclonal antibody, FITC

Applications Flow Cytometry.
Reactivities Human, Monkey
Conjugation FITC
Immunogen Native human IgE

Chicken IgA (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. Can be used to prepare conjugates of choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Immunohistochemistry: 1/50-1/250.
Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Antibody titre: Precipitin titre 1/64 when tested against pooled normal chicken serum in agar-block immunodiffusion titration.
Reactivities Chicken
Immunogen Purified normal IgA isolated from pooled Chicken serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Chicken IgA (Fc specific) goat polyclonal antibody, Biotin

Applications Immunocytochemical and Immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates.
To demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases.
Identification of a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure.
Non-isotopic assay methodology (e.g. ELISA) to measure IgA in chicken serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Immunocytochemistry: 1/50-1/250.
Immunohistochemistry: 1/50-1/250.
Reactivities Chicken
Conjugation Biotin
Immunogen Purified normal IgA isolated from pooled Chicken serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Chicken IgA (Fc specific) goat polyclonal antibody, FITC

Applications Immunocytochemical and Immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates.
To demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases.
Identification of a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Immunocytochemistry: 1/10-1/40.
Immunohistochemistry: 1/10-1/40.
Reactivities Chicken
Conjugation FITC
Immunogen Purified IgA isolated from Chicken serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Chicken IgA (Fc specific) goat polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in chicken serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Immunocytochemistry: 1/50-1/250.
Immunohistochemistry: 1/50-1/250.
Reactivities Chicken
Conjugation HRP
Immunogen Purified normal IgA isolated from pooled Chicken serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Chicken IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in chicken serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000 - 1/5000.
Reactivities Chicken
Conjugation Biotin
Immunogen Purified IgM isolated from chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Duck IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
Can be used to prepare conjugates of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/200-1/4,000.
Immunohistochemistry: 1/50-1/250.
Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Antibody titre: Precipitin titre 1/16 when tested against pooled normal Duck serum in agar-block immunodiffusion titration.
Reactivities Duck
Immunogen Purified IgM isolated from Duck serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Duck IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Suitable for use in Immunocytochemical and iImunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in duck serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Immunohistochemistry and Immunocytochemistry: 1/50-1/250.
Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Note: This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Duck
Conjugation Biotin
Immunogen Purified IgM isolated from Duck serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Duck IgM (Fc specific) goat polyclonal antibody, FITC

Applications Suitable for use in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
Recommended Dilutions: 1/10-1/40.
Note: This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Duck
Conjugation FITC
Immunogen Purified normal IgM isolated from pooled Duck serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Guinea Pig IgG2 (subclass specific) goat polyclonal antibody, FITC

Applications Can be used to identify and measure IgG2, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of guinea pig origin in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions: 1/20 - 1/80.
Reactivities Guinea Pig
Conjugation FITC
Immunogen Pools of highly purified homogenous IgG2 isolated from guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Guinea Pig IgM (Fc specific) goat polyclonal antibody, FITC

Applications In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions: 1/10 - 1/40.
Reactivities Guinea Pig
Conjugation FITC
Immunogen Purified IgM isolated from guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgD (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40, depending on the method used.
Reactivities Human
Conjugation FITC
Immunogen Pools of purified homogenous IgD isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgD (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used in direct Immunofluorescence staining of cytoplasmic IgD of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions: 1/10 - 1/30.
Reactivities Human
Conjugation TRITC
Immunogen Purified monoclonal IgD isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled secondary antibody for Indirect detection of IgE in Human cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in human serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of human cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Human
Immunogen Purified homogenous IgE isolated from Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.