Secondary Antibodies

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Mouse IgE (Fc specific) rabbit polyclonal antibody, TRITC

Applications Can be used to identify and measure IgE, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40.
Reactivities Mouse
Conjugation TRITC
Immunogen Pools of highly purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgG (Fab specific) rabbit polyclonal antibody, TRITC

Applications Can be used for direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:10 and 1:40.
Reactivities Monkey
Conjugation TRITC
Immunogen Purified Fab of normal IgG isolated form Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgM (Fc specific) rabbit polyclonal antibody, TRITC

Applications ELISA. 
Immunocytochemistry.  
(In)direct immunofluorescence

Immunohistochemistry on Frozen Sections. 
Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/10- 1/80.
Reactivities Mouse
Conjugation TRITC
Immunogen Purified homogenous IgM isolated from Mouse serum. Immunization with intact and split IgM.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Porcine IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications ELISA. 
Immunocytochemistry. 
Immunofluorescence.
Immunohistochemistry Frozen Sections.

Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two Nordic immunoconjugates with different labels but originating from the same host animal species are used for direct two-colour staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions1/20-1/80.
Reactivities Porcine
Conjugation TRITC
Immunogen Purified normal IgG isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for ELISA, Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA:  1/10,000 - 1/50,000.
IF Microscopy:  1/1000 - 1/5000.
Conjugation TRITC
Immunogen Goat IgG whole molecule.

Guinea pig IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Conjugation TRITC
Immunogen Guinea Pig IgG whole molecule.

Mouse IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilution(s):
FLISA : 1:10,000 - 1:50,000
IF Microscopy : 1:1,000 - 1:5,000
Other Dilution: FLOW CYTOMETRY 1:500 - 1:2,500
Conjugation TRITC
Immunogen Mouse IgG whole molecule.

Goat IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/1,000-1/5,000) and Flow Cytometry (1/500-1/2,500) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Goat IgG, whole molecule

Donkey IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Donkey IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Chicken IgG whole molecule

Chicken IgG / Chicken IgY (Fc specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Chicken IgG F(c) fragment.

Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1:10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Chicken IgG F(ab')2 fragment.

Bovine IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation TRITC
Immunogen Bovine IgG whole molecule.

Bovine IgG (Fc specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation TRITC
Immunogen Bovine IgG F(c) fragment.

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation TRITC
Immunogen Bovine IgG F(ab')2 fragment.

Feline IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Cat IgG F(c) fragment.

Feline IgG (Fc specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Cat IgG F(c) fragment.

Feline IgG F(ab')2 specific rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Cat IgG F(ab’)2 fragment.

Canine IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1:500-1:2,500) and Flow cytometry (1/2,000-1:10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Dog IgG whole molecule.

Canine IgG (Fc specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Dog IgG F(c) fragment.

Canine IgG (F(ab)2 specific) rabbit polyclonal antibody, TRITC

Applications FLISA: 1/10000 - 1/50000.
Immunofluorescence: 1/1000 - 1/5000.
Flow Cytometry: 1/500 - 1/2500.
Conjugation TRITC
Immunogen Dog IgG F(ab')2 fragment

Goat IgG (Fc specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Goat IgG F(c) fragment.

Goat IgG (F(ab)2 specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Goat IgG F(ab')2 fragment.

Goat IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry or FACS analysis (1/2,000-1/10,000) as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Goat IgG F(ab')2 fragment.

Guinea pig IgG (Fc specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Guinea Pig IgG F(c) fragment

Guinea pig IgG F(ab')2 specific rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Guinea Pig IgG F(ab')2 fragment.

Hamster IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Hamster IgG whole molecule.

Golden Syrian Hamster IgG (F(ab)2 specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Hamster IgG F(ab')2 fragment.

Equine IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Horse IgG whole molecule.

Equine IgG rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Horse IgG F(c) fragment.

Human IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Human IgG, whole molecule.

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Human IgG whole molecule.

Human IgG (gamma chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. 
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions: 
FLISA: 1/10,000-1/50,000. 
IF Microscopy: 1/1,000-1/5,000. 
Flow Cytometry: 1/500-1/2,500.
Conjugation TRITC
Immunogen Human IgG heavy chain

Human IgG (Fc specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Human IgG F(c) fragment.

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1:500-1:2,500) and Flow cytometry (1:2,000-1:10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Human IgG F(ab')2 fragment.

Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Human IgG F(ab')2 fragment.

Human IgM (Fc5 mu specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Human IgM (Fc5µ) fragment

Human IgA (alpha chain) rabbit polyclonal antibody, TRITC

Applications This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Conjugation TRITC
Immunogen Human IgA alpha chain.

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgG whole molecule.

Mouse IgG (gamma chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgG heavy chain.

Mouse IgG (Fc specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgG F(c) fragment.

Mouse IgG (F(ab)2 specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgG F(ab')2 fragment.

Mouse IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgG F(ab')2 fragment.

Mouse IgM (Mu chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgM mu heavy chain.

Mouse IgA (alpha chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgA alpha chain.

Mouse Kappa Light Chain rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse κ (kappa) light chain fragment.

Mouse lambda light chain rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse lambda light chain.

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgG1 γ1 chain.

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy, Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgG2a γ2a chain specific.

Mouse IgG2b (gamma 2b chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgG2b γ2b chain.