Sheep IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP
Applications | Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:42,000 of the reconstitution concentration is suggested for this product. Western blotting: 1:500--1:3,000. Immunohistochemistry: 1:250-1:1,500. |
Conjugation | HRP |
Immunogen | Sheep IgG whole molecules. |