Secondary Antibodies

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Rabbit Anti-Mouse IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Mouse IgG
Conjugation Unconjugated

Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Goat IgG
Conjugation HRP

Rabbit Anti-Goat IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Goat IgG
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human IgG4 Antibody, Clone RM120

Applications ELISA: 50ng/well - 200ng/well for Capture;, 0.05ug/mL - 0.2ug/mL for Detection;, , WB: 0.5 ug/mL - 2 ug/mL;, ICC: 1 ug/mL-10ug/mL;, IHC: 1 ug/mL-10ug/mL
Reactivities Human IgG4
Conjugation Unconjugated

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, FITC

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. It is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Conjugation FITC

Mouse IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for ELISA, Immunofluorescence Microscopy.
Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1000-1/5000.
Conjugation FITC

Biotin Conjugated Rabbit Anti-Goat IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Goat IgG
Conjugation Biotin

Rabbit Monoclonal Anti-Mouse IgG1 antibody, clone RM106

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB: 0.1ug/mL-0.5ug/mL
Reactivities Mouse IgG1
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human IgG1 Antibody, Clone RM117

Applications ELISA: 50ng/well - 200ng/well for Capture;, 0.05ug/mL - 0.2ug/mL for Detection;, ICC: 0.5ug/mL-2ug/mL, IHC: 0.5ug/mL-2ug/mL
Reactivities Human IgG1
Conjugation Unconjugated

Mouse IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA : 1:50,000 - 1:300,000
Western Blot : 1:10,000 - 1:30,000
Immunohistochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Conjugation HRP

Goat IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA: 1/50,000-1/100,000. 
Western Blot: 1/5,000-1/20,000. 
Immunohistochemistry: 1/1,000-1/5,000. 
Note: HRP Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Conjugation HRP

Rabbit Anti-Human IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Human IgG
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human IgM antibody, clone RM121

Applications ELISA: 50ng/well – 200ng/well (for Capture);0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human IgM
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human IgA antibody, clone RM128

Applications ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human IgA
Conjugation Unconjugated

Monkey IgG (Fab specific) rabbit polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
Reactivities Monkey
Conjugation FITC

Cy3 Conjugated Rabbit Anti-Goat IgG

Applications IF/ICC: 1:50-100; IHC: 1:50-100
Reactivities Goat IgG
Conjugation Cy3

Canine IgG2 rabbit polyclonal antibody, Purified

Applications ELISA: 1:100 - 1:10,000.
Western Blot: 1:1000 - 1:10,000.
Immunohistochemistry on frozen sections: 1:200 - 1:2000.
Reactivities Canine

Peroxidase Conjugated Rabbit Anti-Rat IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rat IgG
Conjugation HRP

Monkey IgG (Fab specific) rabbit polyclonal antibody, HRP

Applications Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells.
Combinations of isotype-specific reagents should be used instead for this purpose.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommneded Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/10000.
Reactivities Monkey
Conjugation HRP

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/75,000-1/500,000.
Western Blot: 1/10,000-1/50,000.
Immunohistochemistry: 1/1,000-1/10,000.

Human IgG (gamma chain specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.

FITC Conjugated Rabbit Anti-Goat IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Goat IgG
Conjugation FITC

Monkey IgG (Fab specific) rabbit polyclonal antibody, Azide Free

Applications Can be used for indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Monkey

Monkey IgG (Fab specific) rabbit polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining to identify and measure free or Ig bound subunits of both kappa and lambda type at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Monkey
Conjugation Biotin

Biotin Conjugated Rabbit Anti-Rat IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Rat IgG
Conjugation Biotin

Rabbit Monoclonal Anti-Mouse IgG (gamma-Chain) antibody, clone RM104

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB (non-reduced): 0.1ug/mL-0.5ug/mL
Reactivities Mouse IgG gamma
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human Gamma Heavy Chain antibody, clone RMG02

Applications ELISA: 0.05ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL
Reactivities Human gamma heavy chain
Conjugation Unconjugated

Mouse IgE (Fc specific) rabbit polyclonal antibody, Azide Free

Applications Can be used as unlabelled secondary antibody for indirect detection of IgE in Mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in Mouse serum or other body fluid. When applied in any Immunocytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse

Mouse IgE (Fc specific) rabbit polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/3000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Conjugation Biotin

Mouse IgE (Fc specific) rabbit polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000.
Reactivities Mouse
Conjugation HRP

Bovine IgM (Fc specific) rabbit polyclonal antibody, Biotin

Applications Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/100-1/500.
Reactivities Bovine
Conjugation Biotin

Bovine IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000.
Reactivities Bovine
Conjugation HRP

Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, Biotin

Applications Can be usde in immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2000 - 1/20000.
Reactivities Monkey
Conjugation Biotin

Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, FITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
Reactivities Monkey
Conjugation FITC

Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in monkey serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2000-1/8000.
Reactivities Monkey
Conjugation HRP

Monkey IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
Histochemical and cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2000 - 1/10000.
Reactivities Monkey
Conjugation Biotin

Monkey IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in monkey serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical and cytochemical: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000.
Reactivities Monkey
Conjugation HRP

Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and cytochemical Use: 1/100- 1/2500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000.
Reactivities Mouse
Conjugation Biotin

Mouse IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot. 
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections. 
In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions 
Histochemical and cytochemical Use: 1/100- 1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000- 1/5,000.
Reactivities Mouse
Conjugation HRP

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/500,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/5,000.
Conjugation Biotin

Bovine IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western blot: 1/1,000-1/10,000 or Dot blot), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation HRP

Rat IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidas antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/170,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500- 1/2,500.
Conjugation HRP

Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Goat IgG
Conjugation HRP

Rabbit Monoclonal Anti-Mouse IgG2a Kappa antibody, clone RM107

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB (non-reduced): 0.1ug/mL-0.5ug/mL
Reactivities Mouse IgG2a kappa
Conjugation Unconjugated

Rabbit Monoclonal Anti-Mouse IgG2b (gamma-2b) antibody, clone RM108

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB (stronger on non-reduced): 0.1ug/mL-0.5ug/mL
Reactivities Mouse IgG2b
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human Kappa Light Chain antibody, clone RM126

Applications ELISA: 0.05ug/mL-0.2ug/ml; ICC/IHC: 0.5ug/mL-2ug/mL
Reactivities Human kappa light chain
Conjugation Unconjugated

Mouse IgE (Fc specific) rabbit polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgE in mouse serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80, depending on the method used.
Reactivities Mouse
Conjugation FITC

Mouse IgE (Fc specific) rabbit polyclonal antibody, TRITC

Applications Can be used to identify and measure IgE, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40.
Reactivities Mouse
Conjugation TRITC

Bovine IgM (Fc specific) rabbit polyclonal antibody, Azide Free

Applications This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase Immunochemistry.
Recommneded Dilutions:
Histochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000.
Reactivities Bovine

Bovine IgM (Fc specific) rabbit polyclonal antibody, FITC

Applications Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
Recommneded Dilutions: 1/20-1/80.
Reactivities Bovine
Conjugation FITC