Secondary Antibodies

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Bovine IgG (H+L chain) Rabbit Polyclonal Antibody
R1304HRP

USD 255.00

3 Days

Bovine IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western blot: 1/1,000-1/10,000 or Dot blot), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation HRP
Immunogen Bovine IgG whole molecule.
Rat IgG (H+L chain) Rabbit Polyclonal Antibody
R1371HRP

USD 240.00

3 Days

Rat IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidas antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/170,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500- 1/2,500.
Conjugation HRP
Immunogen Rat IgG whole molecule
Peroxidase Conjugated Goat Anti-Mouse IgM (u-chain specific)
TA130007

USD 225.00

In Stock

Peroxidase Conjugated Goat Anti-Mouse IgM (u-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Mouse IgM
Conjugation HRP
Immunogen Mouse IgM, whole molecule
Biotin Conjugated Goat Anti-Mouse IgG
TA130009

USD 170.00

3 Weeks

Biotin Conjugated Goat Anti-Mouse IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Mouse IgG
Conjugation Biotin
Immunogen Mouse IgG, whole molecule
Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)
TA130032

USD 295.00

3 Weeks

Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Goat IgG
Conjugation HRP
Immunogen Goat IgG, whole molecule
Human IgE Goat Polyclonal Antibody
AP05393FC-N

USD 355.00

5 Days

Human IgE goat polyclonal antibody, FITC

Applications Flow Cytometry.
Reactivities Human, Monkey
Conjugation FITC
Immunogen Native human IgE
Sheep/Goat IgG Donkey Polyclonal Antibody
AP05395HR-N

USD 735.00

5 Days

Sheep/Goat IgG donkey polyclonal antibody, HRP

Applications ELISA: 1/12800-1/25600.
Western Blot: 1/20000.
Immunohistochemistry on Paraffin Sections.
Immunohistochemistry on Frozen Sections: 1/50-1/100.
Reactivities Goat, Sheep
Conjugation HRP
Immunogen Sheep IgG
Chicken IgA (Fc specific) Goat Polyclonal Antibody
AP21442AF-N

USD 470.00

2 Weeks

Chicken IgA (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. Can be used to prepare conjugates of choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Immunohistochemistry: 1/50-1/250.
Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Antibody titre: Precipitin titre 1/64 when tested against pooled normal chicken serum in agar-block immunodiffusion titration.
Reactivities Chicken
Immunogen Purified normal IgA isolated from pooled Chicken serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Chicken IgA (Fc specific) Goat Polyclonal Antibody
AP21442BT-N

USD 410.00

2 Weeks

Chicken IgA (Fc specific) goat polyclonal antibody, Biotin

Applications Immunocytochemical and Immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates.
To demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases.
Identification of a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure.
Non-isotopic assay methodology (e.g. ELISA) to measure IgA in chicken serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Immunocytochemistry: 1/50-1/250.
Immunohistochemistry: 1/50-1/250.
Reactivities Chicken
Conjugation Biotin
Immunogen Purified normal IgA isolated from pooled Chicken serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Chicken IgA (Fc specific) Goat Polyclonal Antibody
AP21442FC-N

USD 410.00

2 Weeks

Chicken IgA (Fc specific) goat polyclonal antibody, FITC

Applications Immunocytochemical and Immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates.
To demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases.
Identification of a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Immunocytochemistry: 1/10-1/40.
Immunohistochemistry: 1/10-1/40.
Reactivities Chicken
Conjugation FITC
Immunogen Purified IgA isolated from Chicken serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Chicken IgA (Fc specific) Goat Polyclonal Antibody
AP21442HR-N

USD 410.00

2 Weeks

Chicken IgA (Fc specific) goat polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in chicken serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Immunocytochemistry: 1/50-1/250.
Immunohistochemistry: 1/50-1/250.
Reactivities Chicken
Conjugation HRP
Immunogen Purified normal IgA isolated from pooled Chicken serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Chicken IgM (Fc specific) Goat Polyclonal Antibody
AP21443BT-N

USD 410.00

2 Weeks

Chicken IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in chicken serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000 - 1/5000.
Reactivities Chicken
Conjugation Biotin
Immunogen Purified IgM isolated from chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Duck IgM (Fc specific) Goat Polyclonal Antibody
AP21447AF-N

USD 470.00

2 Weeks

Duck IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
Can be used to prepare conjugates of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/200-1/4,000.
Immunohistochemistry: 1/50-1/250.
Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Antibody titre: Precipitin titre 1/16 when tested against pooled normal Duck serum in agar-block immunodiffusion titration.
Reactivities Duck
Immunogen Purified IgM isolated from Duck serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Duck IgM (Fc specific) Goat Polyclonal Antibody
AP21447BT-N

USD 550.00

2 Weeks

Duck IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Suitable for use in Immunocytochemical and iImunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in duck serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Immunohistochemistry and Immunocytochemistry: 1/50-1/250.
Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Note: This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Duck
Conjugation Biotin
Immunogen Purified IgM isolated from Duck serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Duck IgM (Fc specific) Goat Polyclonal Antibody
AP21447FC-N

USD 410.00

2 Weeks

Duck IgM (Fc specific) goat polyclonal antibody, FITC

Applications Suitable for use in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
Recommended Dilutions: 1/10-1/40.
Note: This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Duck
Conjugation FITC
Immunogen Purified normal IgM isolated from pooled Duck serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Guinea Pig IgG2 (subclass specific) Goat Polyclonal Antibody
AP21454FC-N

USD 550.00

2 Weeks

Guinea Pig IgG2 (subclass specific) goat polyclonal antibody, FITC

Applications Can be used to identify and measure IgG2, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of guinea pig origin in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions: 1/20 - 1/80.
Reactivities Guinea Pig
Conjugation FITC
Immunogen Pools of highly purified homogenous IgG2 isolated from guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Guinea Pig IgG2 (subclass specific) Goat Polyclonal Antibody
AP21454SU-N

USD 270.00

2 Weeks

Guinea Pig IgG2 (subclass specific) goat polyclonal antibody, Serum

Applications Immunoprecipitation.
Intended for use in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2 in guinea pig serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of guinea pig IgG2 from serum or plasma. 
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Precipitin titre: 1/32 when tested against pooled normal Guinea Pig serum in agar-block Immunodiffusion titration.
Reactivities Guinea Pig
Immunogen Pools of purified normal IgG2 isolated from pooled Guinea Pig serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Guinea Pig IgM (Fc specific) Goat Polyclonal Antibody
AP21455FC-N

USD 410.00

2 Weeks

Guinea Pig IgM (Fc specific) goat polyclonal antibody, FITC

Applications In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions: 1/10 - 1/40.
Reactivities Guinea Pig
Conjugation FITC
Immunogen Purified IgM isolated from guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgD (Fc specific) Goat Polyclonal Antibody
AP21479FC-N

USD 410.00

2 Weeks

Human IgD (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40, depending on the method used.
Reactivities Human
Conjugation FITC
Immunogen Pools of purified homogenous IgD isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgD (Fc specific) Goat Polyclonal Antibody
AP21479TC-N

USD 410.00

2 Weeks

Human IgD (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used in direct Immunofluorescence staining of cytoplasmic IgD of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions: 1/10 - 1/30.
Reactivities Human
Conjugation TRITC
Immunogen Purified monoclonal IgD isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgE (Fc specific) Goat Polyclonal Antibody
AP21480AF-N

USD 320.00

2 Weeks

Human IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled secondary antibody for Indirect detection of IgE in Human cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in human serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of human cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Human
Immunogen Purified homogenous IgE isolated from Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgE (Fc specific) Goat Polyclonal Antibody
AP21480TC-N

USD 410.00

2 Weeks

Human IgE (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used for direct Immunofluorescence staining of cytoplasmic IgE of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/30.
Reactivities Human
Conjugation TRITC
Immunogen Purified monoclonal IgE isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgD (Fc specific) Goat Polyclonal Antibody
AP21481FC-N

USD 550.00

2 Weeks

Mouse IgD (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgD in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/10-1/40, depending on the method used.
Reactivities Mouse
Conjugation FITC
Immunogen Pools of purified homogenous IgD isolated from BALB/C and C57Bl Mouse serum. F reund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgD (Fc specific) Goat Polyclonal Antibody
AP21481TC-N

USD 410.00

2 Weeks

Mouse IgD (Fc specific) goat polyclonal antibody, TRITC

Applications In immunocytochemical and immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgD in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40, depending on the method used.
Reactivities Mouse
Conjugation TRITC
Immunogen Pools of purified homogenous IgD isolated from BALB/C and C57Bl mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Goat Polyclonal Antibody
AP21482AF-N

USD 410.00

2 Weeks

Mouse IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled secondary antibody for indirect detection of IgE in mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Immunogen Purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Goat Polyclonal Antibody
AP21482BT-N

USD 410.00

2 Weeks

Mouse IgE (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000.
Reactivities Mouse
Conjugation Biotin
Immunogen Purified homogenous IgE isolated from Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgE (Fc specific) Goat Polyclonal Antibody
AP21483AF-N

USD 470.00

2 Weeks

Rat IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications This antibody can be used:
• As unlabelled secondary antibody for indirect detection of IgE in Rat cell, tissue substrates and body fluids.
• In Immunofluorescence and immunoenzyme assay methods.
• For the production of immunoconjugates with a selected marker.
• To prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier.
• As catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid.
Recommended Dilutions:
Histochemistry and Cytochemistry: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Reactivities Rat
Immunogen Purified homogenous IgE isolated from Rat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgE (Fc specific) Goat Polyclonal Antibody
AP21483FC-N

USD 410.00

2 Weeks

Rat IgE (Fc specific) goat polyclonal antibody, FITC

Applications This antibody can be used
In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To identify and measure IgE in Rat serum or other body fluids.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions: 1/20-1/80.
Reactivities Rat
Conjugation FITC
Immunogen Purified homogenous IgE isolated from Rat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgE (Fc specific) Goat Polyclonal Antibody
AP21483TC-N

USD 410.00

2 Weeks

Rat IgE (Fc specific) goat polyclonal antibody, TRITC

Applications This antibody can be used:
To identify and measure IgE, antigen or antibody, at the cellular and subcellular level by immunofluorescence
staining of appropriately treated cell and tissue substrates.
To identify circulating IgE antibodies in Rat serum or other body fluids.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions: 1/10-1/60.
Reactivities Rat
Conjugation TRITC
Immunogen Purified homogenous IgE isolated from Rat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Rabbit Polyclonal Antibody
AP21485FC-N

USD 550.00

2 Weeks

Mouse IgE (Fc specific) rabbit polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgE in mouse serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80, depending on the method used.
Reactivities Mouse
Conjugation FITC
Immunogen Pools of purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Rabbit Polyclonal Antibody
AP21485TC-N

USD 550.00

2 Weeks

Mouse IgE (Fc specific) rabbit polyclonal antibody, TRITC

Applications Can be used to identify and measure IgE, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40.
Reactivities Mouse
Conjugation TRITC
Immunogen Pools of highly purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rabbit IgM (Fc specific) Goat Polyclonal Antibody
AP21488AF-N

USD 470.00

2 Weeks

Rabbit IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended working dilutions:
Histochemistry: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/3000.
Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration.
Reactivities Rabbit
Immunogen Purified IgM isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rabbit IgM (Fc specific) Goat Polyclonal Antibody
AP21488FC-N

USD 410.00

2 Weeks

Rabbit IgM (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40.
Reactivities Rabbit
Conjugation FITC
Immunogen Purified IgM isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rabbit IgA (Fc specific) Goat Polyclonal Antibody
AP21489AF-N

USD 470.00

2 Weeks

Rabbit IgA (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry.
When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2500.
Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration.
Reactivities Rabbit
Immunogen Purified IgA isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure
Rabbit IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP21490FC-N

USD 410.00

2 Weeks

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, FITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80.
Reactivities Rabbit
Conjugation FITC
Immunogen Purified polyclonal rabbit IgG, and IgA and IgM containing factions isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rabbit IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP21490TC-N

USD 410.00

2 Weeks

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, TRITC

Applications Can be used to identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two immunoconjugates with different labels but originating from the same host animal species are used for direct two-color staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80.
Reactivities Rabbit
Conjugation TRITC
Immunogen Purified immunoglobulin fractions containing IgG, IgA and IgM isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgG2b (subclass specific) Goat Polyclonal Antibody
AP21491AF-N

USD 410.00

2 Weeks

Rat IgG2b (subclass specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended working dilutions:
Histochemistry: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000.
Reactivities Rat
Immunogen Pools of purified homogenous IgG2b isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgG2b (subclass specific) Goat Polyclonal Antibody
AP21491FC-N

USD 410.00

2 Weeks

Rat IgG2b (subclass specific) goat polyclonal antibody, FITC

Applications Can be used to identify and measure IgG2b, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80.
Reactivities Rat
Conjugation FITC
Immunogen Pools of highly purified homogenous IgG2b isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgG2ab (subclass specific) Goat Polyclonal Antibody
AP21493AF-N

USD 410.00

2 Weeks

Rat IgG2ab (subclass specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection of IgG2a at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry.
When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended working dilutions:
Histochemistry: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000.
Reactivities Rat
Immunogen Pools of purified homogenous IgG2a and IgG2b isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgG2ab (subclass specific) Goat Polyclonal Antibody
AP21493BT-N

USD 410.00

2 Weeks

Rat IgG2ab (subclass specific) goat polyclonal antibody, Biotin

Applications Can be used:
In immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level of appropriately treated cell and tissue substrates.
To demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using a reference antibody of Rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids.
As a second step an avidin or streptavidin conjugate of the user's choice has to be used.
This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250. 
ELISA and comparable non-precipitating antibody-binding assays between: 1/2.000-1/10,000.
Reactivities Rat
Conjugation Biotin
Immunogen Pools of highly purified homogenous IgG2a and IgG2b isolated from rat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgG2ab (subclass specific) Goat Polyclonal Antibody
AP21493FC-N

USD 410.00

2 Weeks

Rat IgG2ab (subclass specific) goat polyclonal antibody, FITC

Applications Can be used to identify and measure IgG2, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions: 1/20 - 1/80.
Reactivities Rat
Conjugation FITC
Immunogen Pools of highly purified homogenous IgG2a and IgG2b isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgG2ab (subclass specific) Goat Polyclonal Antibody
AP21493TC-N

USD 410.00

2 Weeks

Rat IgG2ab (subclass specific) goat polyclonal antibody, TRITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgG2 in rat serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions 1/20 - 1/80, depending on the method used.
Reactivities Rat
Conjugation TRITC
Immunogen Pools of purified homogenous IgG2a and IgG2b isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgG3 (subclass specific) Goat Polyclonal Antibody
AP21494AF-N

USD 550.00

2 Weeks

Mouse IgG3 (subclass specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry.
When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended Working Dilutions:
Histochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000.
Performance testing: Titre, specificity and reactivity of the subclass specific cross-adsorbed IgG fraction is further evaluated in a number of highly sensitive non-precipitating antibody-binding assay systems. These performance tests include direct single and double identification of cIg in mouse cell and tissue substrates and their evaluation as second antibody in the analysis of human cells and tissues after reacting with a primary monoclonal mouse antibody to a human antigen. The quantitative specific recognition ability is verified in double staining procedures together with reference reagents of known specificity and reactivity.
Reactivities Mouse
Immunogen Pools of purified homogenous IgG3 isolated from pooled Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgG3 (subclass specific) Goat Polyclonal Antibody
AP21494TC-N

USD 550.00

2 Weeks

Mouse IgG3 (subclass specific) goat polyclonal antibody, TRITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgG3 in mouse serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Working dilutions 1/10 - 1/40, depending on the method used.
Reactivities Mouse
Conjugation TRITC
Immunogen Pools of purified homogenous IgG3 isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Bovine IgM (Fc specific) Rabbit Polyclonal Antibody
AP21498AF-N

USD 470.00

2 Weeks

Bovine IgM (Fc specific) rabbit polyclonal antibody, Azide Free

Applications This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase Immunochemistry.
Recommneded Dilutions:
Histochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000.
Reactivities Bovine
Immunogen Purified normal IgM isolated from pooled Bovine serum.
Feund’s complete adjuvant is used in the first step of the immunization procedure.
Bovine IgM (Fc specific) Rabbit Polyclonal Antibody
AP21498FC-N

USD 550.00

2 Weeks

Bovine IgM (Fc specific) rabbit polyclonal antibody, FITC

Applications Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
Recommneded Dilutions: 1/20-1/80.
Reactivities Bovine
Conjugation FITC
Immunogen Purified normal IgM isolated from pooled Bovine serum.
Feund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
AP31435AF-N

USD 470.00

2 Weeks

Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Azide Free

Applications This IgG fraction allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays are between 1/1000 and 1/5000.
Reactivities Monkey
Immunogen Highly purified IgG and pools of homogenous IgA and IgM isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
AP31435TC-N

USD 410.00

2 Weeks

Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, TRITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/10 and 1/40.
Reactivities Monkey
Conjugation TRITC
Immunogen Purified immunoglobulin fractions containing IgG, IgA and IgM isolated from pooled monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (Fc specific) Goat Polyclonal Antibody
AP31436AF-N

USD 470.00

2 Weeks

Monkey IgA + IgG + IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays are between 1/1000 and 1/5000.
Reactivities Monkey
Immunogen Highly purified immunoglobulin fractions containing IgG, IgA and IgM isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA (Fc specific) Goat Polyclonal Antibody
AP31437AF-N

USD 470.00

2 Weeks

Monkey IgA (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/2500.
Reactivities Monkey
Immunogen Purified IgA isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.