Secondary Antibodies

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Peroxidase Conjugated Goat Anti-Rabbit IgG (Min Hu, Ms)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rabbit IgG
Conjugation HRP
Immunogen Rabbit IgG, whole molecule

Peroxidase Conjugated Affinity Purified Goat anti-Mouse IgG [H&L]

Applications WB: 1: 1,000; ICC: 1:200; IP: 1:200
Reactivities Mouse IgG
Conjugation HRP
Immunogen Mouse IgG, whole molecule

Peroxidase Conjugated Goat Anti-Rabbit IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rabbit IgG
Conjugation HRP
Immunogen Rabbit IgG, whole molecule

Goat Anti-Rabbit IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Rabbit IgG
Conjugation Unconjugated
Immunogen Rabbit IgG, whole molecule

Peroxidase Conjugated Goat Anti-Mouse IgG (gamma-chain specific)

Applications Dot blot:0.5-1µg/ml.Western blot(DAB):0.7-3.3µg/ml.Western blot(ECL):0.2-0.5µg/ml.ELISA:0.2-0.3µg/ml.
Reactivities Mouse IgG
Conjugation HRP
Immunogen Mouse IgG, whole molecule

Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), HRP, Purified

Applications ELISA: 1/4000-1/8000 (See also Ref.2-9).
Applications Reported in Literature:
Flow Cytometry (Ref.15,18,19).
Immunohistochemistry on Frozen Sections (Ref.10).
Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11).
Immunocytochemistry (Ref.12).
Immunoblotting (Ref.3,13-16).
Microarray (Ref.17).
Multiplex (Ref.2,20-22).
Purification (Ref.15).
Reactivities Human
Conjugation HRP

Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), AP, Purified

Applications ELISA: 1/1000-1/2000 (See also Ref.2-9). 
FLISA
Applications Reported in Literature: 
Flow Cytometry (Ref.15,18,19).
Immunohistochemistry on Frozen Sections (Ref.10).
Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11).
Immunocytochemistry (Ref.12).
Immunoblotting (Ref.3,13-16).
Microarray (Ref.17).
Multiplex (Ref.2,20-22).
Purification (Ref.15).
Reactivities Human
Conjugation AP

IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified

Applications ELISA: 1/1000-1/2000 (2-9).
Immunohistochemistry on Frozen Sections:  reported in literature (5,7,10,11).
Immunohistochemistry on Paraffin Sections: reported in literature (10).
Flow Cytometry:
reported in literature (15, 18, 19).
Immunocytochemistry: reported in literature (12).
Western Blot: reported in literature (3,13-16).
Reactivities Human
Conjugation AP

Goat Anti-Mouse IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Mouse IgG
Conjugation Unconjugated
Immunogen Mouse IgG, whole molecule

FITC Conjugated Goat Anti-Rabbit IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Rabbit IgG
Conjugation FITC
Immunogen Rabbit IgG, whole molecule

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Ascites

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200. 
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human

Biotin Conjugated Goat Anti-Rabbit IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Rabbit IgG
Conjugation Biotin
Immunogen Rabbit IgG, whole molecule

Rabbit Anti-Mouse IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Mouse IgG
Conjugation Unconjugated
Immunogen Mouse IgG, whole molecule

Peroxidase Conjugated Goat Anti-Mouse IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Mouse IgG
Conjugation HRP
Immunogen Mouse IgG, whole molecule

Peroxidase Conjugated Goat Anti-Rabbit IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rabbit IgG
Conjugation HRP
Immunogen Rabbit IgG, whole molecule

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Biotin

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
General Recommended Dilutions:
Immunocytochemistry.
Immunohistochemistry (Paraffin).
Histochemical Use: 1/10-1/40.
ELISA: 1/100-1/2000. 
Western blot: 1/200-1/4000.
Reactivities Human
Conjugation Biotin

FITC Conjugated Goat Anti-Mouse IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Mouse IgG
Conjugation FITC
Immunogen Mouse IgG, whole molecule

Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Goat IgG
Conjugation HRP
Immunogen Goat IgG, whole molecule

Rabbit IgG (H+L chain) goat polyclonal antibody, HRP, Lyophilized

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/150,000-1/250,000.
Immunohistochemistry: 1/500-1/2,500.
Western blot: 1/10,000-1/40,000.
Reactivities Rabbit
Conjugation HRP
Immunogen Rabbit IgG, whole molecule

FITC Conjugated Goat Anti-Rabbit IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Rabbit IgG
Conjugation FITC
Immunogen Rabbit IgG, whole molecule

Biotin Conjugated Goat Anti-Mouse IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Mouse IgG
Conjugation Biotin
Immunogen Mouse IgG, whole molecule

Rabbit Anti-Goat IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Goat IgG
Conjugation Unconjugated
Immunogen Goat IgG, whole molecule

Rabbit Monoclonal Anti-Human IgG4 Antibody, Clone RM120

Applications ELISA: 50ng/well - 200ng/well for Capture;, 0.05ug/mL - 0.2ug/mL for Detection;, , WB: 0.5 ug/mL - 2 ug/mL;, ICC: 1 ug/mL-10ug/mL;, IHC: 1 ug/mL-10ug/mL
Reactivities Human IgG4
Conjugation Unconjugated

Biotin Conjugated Goat Anti-Rabbit IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Rabbit IgG
Conjugation Biotin
Immunogen Rabbit IgG, whole molecule

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), HRP

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
ELISA: 1/20-1/500.
Western Blot: 1/40-1/1000. 
Histochemical Use: 1/5-1/20.
Reactivities Human
Conjugation HRP

Rabbit IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions
IF Microscopy.
ELISA: 1/10,000-1/60,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2500.
Immunoprecipitation.
Reactivities Rabbit
Immunogen Rabbit IgG whole molecule

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, HRP

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay.
Reactivities Human
Conjugation HRP

Monkey IgG (Fc specific) goat polyclonal antibody, FITC

Applications Can be used:
• In direct staining of cytoplasmic IgG in fixed Monkey cells and tissue substrates.
• To identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases.
• To identify a specific antigen or immune complex using a reference antibody of Monkey in the middle layer of the test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilution: 1/20-1/80.
Reactivities Monkey
Conjugation FITC
Immunogen Purified normal IgG isolated from pooled Rhesus Monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgG (H+L chain) sheep polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Immunogen Mouse IgG whole molecule.

Human IgG2 (Hinge Region) mouse monoclonal antibody, clone 3C7, Purified

Applications ELISA.
Reactivities Human

Cy3 Conjugated Goat Anti-Rabbit IgG

Applications IF/ICC: 1:50-100; IHC: 1:50-100
Reactivities Rabbit IgG
Conjugation Cy3
Immunogen Rabbit IgG, whole molecule

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, FITC

Applications Flow Cytometry: Use 10 µl of 2.5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

Goat Monoclonal Anti-Mouse IgG Fc Antibody, Clone RMG06

Applications ELISA: 0.05ug/mL - 1ug/mL;, ICC, IHC: 0.5ug/mL - 2ug/mL;, WB: 0.1ug/mL - 0.5ug/mL
Reactivities Mouse IgG
Conjugation Unconjugated

Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, HRP, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on Frozen Sections. 
Reactivities Mouse
Conjugation HRP

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, FITC

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. It is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Conjugation FITC
Immunogen Sheep IgG F(ab')2 fragment.

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, HRP

Applications ELISA: 1/2,000-1/8,000.
Reactivities Human
Conjugation HRP

Human IgM (Mu chain specific) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/80,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Human IgM µ (mu) heavy chain

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, Purified

Applications ELISA: 5 µg/ml. This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent and purified Rat IgA (Cat.-No SA013) as antigen.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Rat

Human IgG (Fc specific) goat polyclonal antibody, HRP

Applications ELISA: 1/3000.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation HRP
Immunogen Human IgG

Human IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1-5 µg/ml.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Immunogen Human IgG

Human IgG3 sheep polyclonal antibody, HRP

Applications ELISA.
Immunohistochemistry on Frozen Sections: 1/100-1/200.
Reactivities Human
Conjugation HRP
Immunogen Human IgG3 (kappa & lambda) purified from Human myeloma serum

Mouse IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for ELISA, Immunofluorescence Microscopy.
Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1000-1/5000.
Conjugation FITC
Immunogen Mouse IgG whole molecule.

Mouse IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000. 
Western blot: 1/2,000-1/10,000. 
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Immunogen Anti-Mouse IgG F(c) fragment was produced by repeated immunization with Mouse IgG F(c) fragment in goat.

Cy3 Conjugated Goat Anti-Mouse IgG

Applications IF/ICC: 1:50-100; IHC: 1:50-100
Reactivities Mouse IgG
Conjugation Cy3
Immunogen Mouse IgG, whole molecule

Rabbit IgG (H+L chain) goat polyclonal antibody, AP, Liquid

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/3,000-1/25,000.
Western blot: 1/1,000-1/4,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Rabbit
Conjugation AP
Immunogen Rabbit IgG, whole molecule.

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, Purified

Applications ELISA: 5µg/ml as coating antibody.
Reactivities Mouse

Biotin Conjugated Goat Anti-Mouse IgM

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Mouse IgM
Conjugation Biotin
Immunogen Mouse IgM, whole molecule

Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified

Applications Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C.
SDS-PAGE (12% separating gel).
Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min.
Strongly reacts with Bovine IgG light chains, weakly reacts with IgM.
Flow Cytometry.
Immunohistochemistry on Frozen Sections.
Reactivities Bovine, Sheep

Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
For use in ELISA a dilution of 1:3,000-1:12,000 is recommended.
Immunogen Mouse IgG whole molecule.

Biotin Conjugated Rabbit Anti-Goat IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Goat IgG
Conjugation Biotin
Immunogen Goat IgG, whole molecule