Secondary Antibodies

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Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), HRP, Purified

Applications ELISA: 1/4000-1/8000 (See also Ref.2-9).
Applications Reported in Literature:
Flow Cytometry (Ref.15,18,19).
Immunohistochemistry on Frozen Sections (Ref.10).
Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11).
Immunocytochemistry (Ref.12).
Immunoblotting (Ref.3,13-16).
Microarray (Ref.17).
Multiplex (Ref.2,20-22).
Purification (Ref.15).
Reactivities Human
Conjugation HRP

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), HRP

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
ELISA: 1/20-1/500.
Western Blot: 1/40-1/1000. 
Histochemical Use: 1/5-1/20.
Reactivities Human
Conjugation HRP

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, HRP

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay.
Reactivities Human
Conjugation HRP

Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, HRP, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on Frozen Sections. 
Reactivities Mouse
Conjugation HRP

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, HRP

Applications ELISA: 1/2,000-1/8,000.
Reactivities Human
Conjugation HRP

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), HRP

Applications To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: from 1/20 upwards.  
ELISA: form 1/50 upwards.  
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation HRP

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, HRP

Applications ELISA: 1/4,000-1/10,000.
Western blotting.
Dot- and Slot-immunoblotting.
Immunohistochemistry on Frozen Sections: 1/10-1/20.
Reactivities Human
Conjugation HRP

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), HRP

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
ELISA: from 1/100 upwards.
Western blot: from 1/250 upwards.
Reactivities Human
Conjugation HRP

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, HRP

Applications ELISA: 500 ng/ml. 
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with SM1491P  as the capture reagent.
Reactivities Mouse
Conjugation HRP

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), HRP

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation HRP

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), HRP

Applications To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.  
ELISA: from 1/500 upwards. 
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation HRP

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, HRP

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/500
ELISA: from 1/1000 upwards
Western blot: from 1/2000 upwards.
Reactivities Human
Conjugation HRP

Human IgM mouse monoclonal antibody, clone NI 179, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation HRP

Human IgA1 mouse monoclonal antibody, clone NI 69-11 (A89-036), HRP

Applications To identify the presence of IgA1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA1, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/50 upwards
Western blot: from 1/100 upwards
Reactivities Human
Conjugation HRP

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), HRP

Applications To identify the presence of IgA2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct Immunoperoxidase staining of cytoplasmic IgA2, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation HRP

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), HRP

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Immunoperoxidase staining of cytoplasmic IgA2(m)2, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/60 upwards.
Western blot: from 1/20 upwards.
Reactivities Human
Conjugation HRP

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), HRP

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation HRP

Human IgD mouse monoclonal antibody, clone NI 158, HRP

Applications To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/500.
ELISA: from 1/1000 upwards.
Western blot: from 1/2000 upwards.
Reactivities Human
Conjugation HRP

Human IgE mouse monoclonal antibody, clone NI 307, HRP

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation HRP

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), HRP

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct Immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/25-1/100.
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation HRP

Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, HRP

Applications ELISA: 0.1-0.5 µg/ml. 
Western blot.
Reactivities Human
Conjugation HRP

Human IgM mouse monoclonal antibody, clone ICL-931, HRP, Purified

Applications ELISA: 1/1000.
Radioimmunoassay.
Dot Blot: 1/100.
Immunohistochemistry on paraffin sections: 1/40.
Reactivities Human
Conjugation HRP

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-9, HRP

Applications ELISA (250 ng/ml).
Reactivities Mouse
Conjugation HRP

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, HRP

Applications ELISA: 1/2,000-1/8,000.
Reactivities Human
Conjugation HRP

Human IgG mouse monoclonal antibody, clone EFE-565, HRP, Purified

Applications Dot Blot.
ELISA.
Radioimmunoassay.
Immunohistochemistry on Frozen Sections: 1/40.
Reactivities Human
Conjugation HRP

Human IgA mouse monoclonal antibody, clone BFG-267, HRP, Purified

Applications ELISA: 1/1000.
Radioimmunoassay.
Dot Blot: 1/100.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation HRP

Human IgA2 mouse monoclonal antibody, clone A9604D2, HRP

Applications ELISA: 1/500-1/2000.
Reactivities Human
Conjugation HRP

Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, HRP

Applications ELISA: 1/4,000-1/8,000.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG mouse monoclonal antibody, clone 2A9, HRP

Applications ELISA: 1/4,000-1/8,000.
Reactivities Rabbit
Conjugation HRP

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, HRP

Applications ELISA: 1/2,000-1/8,000.
Reactivities Human
Conjugation HRP

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, HRP

Applications ELISA: 1/4,000-1/8,000 (See also Ref.1).
Reactivities Human
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-2, HRP, Aff - Purified

Reactivities Mouse
Conjugation HRP

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, HRP, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on frozen sections. 
Reactivities Mouse
Conjugation HRP

Mouse IgG3 (heavy chain) rat monoclonal antibody, clone LO-MG3-7, HRP, Purified

Applications Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic) and immunohistochemistry on frozen sections.
Reactivities Mouse
Conjugation HRP

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, HRP

Applications Useful as second antibody in Immunoassays, such as ELISA's (good binding on plastic) and Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation HRP

Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-9, HRP, Purified

Applications

Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic),
immunohistochemistry on frozen sections and immunoaffinity chromatography.

Reactivities Mouse
Conjugation HRP

Rat IgE mouse monoclonal antibody, clone MARE-1, HRP

Applications ELISA: 0.5 µg/ml.
Western Blot.
Reactivities Rat
Conjugation HRP

Human IgE mouse monoclonal antibody, clone 1A2, HRP

Applications ELISA: 1/500-1/1,000.
Can be used as a Detection antibody in Sandwich ELISA (paired with 4C3).
Reactivities Human
Conjugation HRP

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, HRP

Applications ELISA: 1/500-1/2000.
Reactivities Human
Conjugation HRP

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, HRP

Applications ELISA: 1/1000-1/2000.
Reactivities Mouse
Conjugation HRP

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone SB73a, HRP

Applications ELISA: 1/2000-1/4000.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation HRP

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, HRP

Applications ELISA: 1/2000-1/4000.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation HRP

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, HRP

Applications ELISA: 1/1000-1/2000.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation HRP

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, HRP

Applications ELISA: 1/1000-1/2000.
Reactivities Mouse
Conjugation HRP