Secondary Antibodies

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Rabbit IgA (Fc specific) Goat Polyclonal Antibody
AP21489BT-N

USD 550.00

2 Weeks

Rabbit IgA (Fc specific) goat polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in rabbit serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/100 - 1/1000.
Reactivities Rabbit
Conjugation Biotin
Immunogen Purified polyclonal IgA isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rabbit IgA (Fc specific) Goat Polyclonal Antibody
AP21489FC-N

USD 410.00

2 Weeks

Rabbit IgA (Fc specific) goat polyclonal antibody, FITC

Applications In immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80.
Reactivities Rabbit
Conjugation FITC
Immunogen Pools of purified IgA isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rabbit IgA (Fc specific) Goat Polyclonal Antibody
AP21489HR-N

USD 550.00

2 Weeks

Rabbit IgA (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in rabbit serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000.
Reactivities Rabbit
Conjugation HRP
Immunogen Purified normal IgA isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rabbit IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP21490AF-N

USD 470.00

2 Weeks

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Azide Free

Applications The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Reactivities Rabbit
Immunogen Highly purified IgG and pools of homogenous IgA and IgM isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgG2b (subclass specific) Goat Polyclonal Antibody
AP21491HR-N

USD 410.00

2 Weeks

Rat IgG2b (subclass specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in Rat serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
Reactivities Rat
Conjugation HRP
Immunogen Pools of purified homogenous IgG2b isolated from Rat sera.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgG2ab (subclass specific) Goat Polyclonal Antibody
AP21493HR-N

USD 410.00

2 Weeks

Rat IgG2ab (subclass specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000 - 1/10,000.
Reactivities Rat
Conjugation HRP
Immunogen Pools of purified homogenous IgG2a and IgG2b isolated from rat sera. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgG3 (subclass specific) Goat Polyclonal Antibody
AP21494BT-N

USD 550.00

2 Weeks

Mouse IgG3 (subclass specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1.000 - 1/5,000.
Reactivities Mouse
Conjugation Biotin
Immunogen Pools of purified homogenous IgG3 isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgG3 (subclass specific) Goat Polyclonal Antibody
AP21494FC-N

USD 550.00

2 Weeks

Mouse IgG3 (subclass specific) goat polyclonal antibody, FITC

Applications This antibody can be used to identify and measure IgG3, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions: 1/20-1/80.
Reactivities Mouse
Conjugation FITC
Immunogen Pools of purified homogenous IgG3 isolated from pooled Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgG3 (subclass specific) Goat Polyclonal Antibody
AP21494HR-N

USD 550.00

2 Weeks

Mouse IgG3 (subclass specific) goat polyclonal antibody, HRP

Applications This antibody can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates To demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in Mouse serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical/Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/7,000 
depending on the method used. Working dilutions schould be stored at +4°C, not refrozen, and preferably used the same day.
Reactivities Mouse
Conjugation HRP
Immunogen Pools of purified homogenous IgG3 isolated from pooled Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Bovine IgM (Fc specific) Rabbit Polyclonal Antibody
AP21498BT-N

USD 550.00

2 Weeks

Bovine IgM (Fc specific) rabbit polyclonal antibody, Biotin

Applications Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/100-1/500.
Reactivities Bovine
Conjugation Biotin
Immunogen Purified normal IgM isolated from pooled Bovine serum.
Feund’s complete adjuvant is used in the first step of the immunization procedure.
Bovine IgM (Fc specific) Rabbit Polyclonal Antibody
AP21498HR-N

USD 550.00

2 Weeks

Bovine IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000.
Reactivities Bovine
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled Bovine serum.
Feund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
AP31435BT-N

USD 550.00

2 Weeks

Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/2000 and 1/10000.
Reactivities Monkey
Conjugation Biotin
Immunogen Highly purified IgG and pools of homogenous IgA and IgM isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
AP31435HR-N

USD 550.00

2 Weeks

Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/2000 and 1/10000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (Fc specific) Goat Polyclonal Antibody
AP31436BT-N

USD 550.00

2 Weeks

Monkey IgA + IgG + IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in monkey serum or other body fluids. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/2000 and 1/10000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified normal IgG , IgA and IgM isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (Fc specific) Goat Polyclonal Antibody
AP31436FC-N

USD 550.00

2 Weeks

Monkey IgA + IgG + IgM (Fc specific) goat polyclonal antibody, FITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/120.
Reactivities Monkey
Conjugation FITC
Immunogen Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (Fc specific) Goat Polyclonal Antibody
AP31436HR-N

USD 550.00

2 Weeks

Monkey IgA + IgG + IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/8000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA (Fc specific) Goat Polyclonal Antibody
AP31437BT-N

USD 550.00

2 Weeks

Monkey IgA (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/4000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified polyclonal IgA isolated from pooled Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA (Fc specific) Goat Polyclonal Antibody
AP31437FC-N

USD 410.00

2 Weeks

Monkey IgA (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80, depending on the method used.
Reactivities Monkey
Conjugation FITC
Immunogen Pools of purified IgA isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA (Fc specific) Goat Polyclonal Antibody
AP31437HR-N

USD 550.00

2 Weeks

Monkey IgA (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in monkey serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/10000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified normal IgA isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (Fc specific) Goat Polyclonal Antibody
AP31438AF-N

USD 470.00

2 Weeks

Monkey IgG (Fc specific) goat polyclonal antibody, Azide Free

Applications As Unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry.
When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended Working Dilutions:
Histochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. 
Antibody Titre: Precipitin titre not less than 1/32 when tested against pooled normal Rhesus Monkey serum in agar-block immunodiffusion titration.
Reactivities Monkey
Immunogen Purified normal IgG isolated from pooled Rhesus Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (Fc specific) Goat Polyclonal Antibody
AP31438BT-N

USD 550.00

2 Weeks

Monkey IgG (Fc specific) goat polyclonal antibody, Biotin

Applications This antibody can be used in Immunocytochemical and Immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates.
To demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases, To identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG in Monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions
Histochemical and Cytochemical Use:  1/100-1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/20000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled Rhesus Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (H+L chain) Goat Polyclonal Antibody
AP31439BT-N

USD 290.00

2 Weeks

Monkey IgG (H+L chain) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (H+L chain) Goat Polyclonal Antibody
AP31439HR-N

USD 290.00

2 Weeks

Monkey IgG (H+L chain) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of antigens or antibodies at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA, Western blotting). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/2000 and 1/10000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled Rhesus Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgM (Fc specific) Goat Polyclonal Antibody
AP31440BT-N

USD 410.00

2 Weeks

Monkey IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and cytochemical use are usually between 1/100 and 1/250.
ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/3000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgM (Fc specific) Goat Polyclonal Antibody
AP31440FC-N

USD 410.00

2 Weeks

Monkey IgM (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working dilutions are usually between 1/20 and 1/80, depending on the method used.
Reactivities Monkey
Conjugation FITC
Immunogen Purified IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgM (Fc specific) Goat Polyclonal Antibody
AP31440HR-N

USD 550.00

2 Weeks

Monkey IgM (Fc specific) goat polyclonal antibody, HRP

Applications ELISA. 
Dot blot. 
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
In Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of Monkey origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in Monkey serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled rhesus monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (H+L chain) Rabbit Polyclonal Antibody
AP31447BT-N

USD 550.00

2 Weeks

Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, Biotin

Applications Can be usde in immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2000 - 1/20000.
Reactivities Monkey
Conjugation Biotin
Immunogen Highly purified IgG, IgA and IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (H+L chain) Rabbit Polyclonal Antibody
AP31447FC-N

USD 550.00

2 Weeks

Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, FITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
Reactivities Monkey
Conjugation FITC
Immunogen Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from Rhesus monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (H+L chain) Rabbit Polyclonal Antibody
AP31447HR-N

USD 550.00

2 Weeks

Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in monkey serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2000-1/8000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified immunoglobulin IgG, IgA and IgM fractions isolated from pooled rhesus monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (H+L chain) Rabbit Polyclonal Antibody
AP31448BT-N

USD 290.00

2 Weeks

Monkey IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
Histochemical and cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2000 - 1/10000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (H+L chain) Rabbit Polyclonal Antibody
AP31448HR-N

USD 290.00

2 Weeks

Monkey IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in monkey serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical and cytochemical: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000.
Reactivities Monkey
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled Rhesus Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA (Secretory component) Goat Polyclonal Antibody
AP31450BT-N

USD 550.00

2 Weeks

Monkey IgA (Secretory component) goat polyclonal antibody, Biotin

Applications Tested in Immunoelectrophoresis, Double Radial Immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes.
Can be used in Immunocytochemical and Immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in Monkey body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/200. 
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/1,000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified free monkey secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA (Secretory component) Goat Polyclonal Antibody
AP31450FC-N

USD 550.00

2 Weeks

Monkey IgA (Secretory component) goat polyclonal antibody, FITC

Applications Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes.
Can be used as reagent for the direct detection of secretory component in monkey cells, tissues and body fluids in immunofluorescence; as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA).
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80.
Reactivities Chimpanzee, Monkey
Conjugation FITC
Immunogen Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Purified free monkey secretory component isolated from pooled milk is used for immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA (Secretory component) Goat Polyclonal Antibody
AP31450HR-N

USD 550.00

2 Weeks

Monkey IgA (Secretory component) goat polyclonal antibody, HRP

Applications Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes.
In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in monkey milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working dilutions:
Histochemical and cytochemical 1/150 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays 1/200 -1/4000.
Reactivities Chimpanzee, Monkey
Conjugation HRP
Immunogen Secretory component can be present in monkey secretions bound to secretory IgA (sIgA) and in free form. Free monkey secretory component isolated from pooled milk is used for immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA (Secretory component) Goat Polyclonal Antibody
AP31450TC-N

USD 550.00

2 Weeks

Monkey IgA (Secretory component) goat polyclonal antibody, TRITC

Applications Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes.
Can be used as reagent for the direct detection of secretory component in monkey cells, tissues and body fluids in immunofluorescence; as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA).
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:10 and 1:40.
Reactivities Chimpanzee, Monkey
Conjugation TRITC
Immunogen Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Purified free monkey secretory component isolated from pooled milk is used for immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgA (Fc specific) Goat Polyclonal Antibody
AP31539HR-N

USD 410.00

2 Weeks

Canine IgA (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in dog serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000.
Reactivities Canine
Conjugation HRP
Immunogen Purified normal IgA isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgG (Fc specific) Goat Polyclonal Antibody
AP31540HR-N

USD 410.00

2 Weeks

Canine IgG (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in Dog serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2000-1/10000.
Reactivities Canine
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled Dog serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgG (H+L chain) Goat Polyclonal Antibody
AP31541BT-N

USD 290.00

2 Weeks

Canine IgG (H+L chain) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of dog origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/10000.
Reactivities Canine
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgG (H+L chain) Goat Polyclonal Antibody
AP31541HR-N

USD 290.00

2 Weeks

Canine IgG (H+L chain) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in dog serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/10000.
Reactivities Canine
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgM (Fc specific) Goat Polyclonal Antibody
AP31542HR-N

USD 550.00

2 Weeks

Canine IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in dog serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/2000.
Reactivities Canine
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543HR-N

USD 550.00

2 Weeks

Mouse IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/400.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/8000 depending on the method used.
Reactivities Mouse
Conjugation HRP
Immunogen Purified homogenous IgM isolated from pooled mouse serum. Immunization with intact (19S) and split IgM (7S). Freund’s complete adjuvant is used in the first step of the immunization procedure.
Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody
AP31795HR-N

USD 260.00

2 Weeks

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP

Applications Sandwich ELISA: 1/120,000 dilution of antibody was found to generate an O.D of 1.0 in a 15 minute reaction with Tetramethyl Benzidine as the substrate.
Western blot (1/5000).
Immunoelectrophoresis.
Reactivities Chicken
Conjugation HRP
Immunogen Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.
Mouse IgM (Fc specific) Rabbit Polyclonal Antibody
AP32992BT-N

USD 550.00

2 Weeks

Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and cytochemical Use: 1/100- 1/2500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000.
Reactivities Mouse
Conjugation Biotin
Immunogen Purified homogenous IgM isolated from Mouse serum. Immunization with intact and split IgM.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Rabbit Polyclonal Antibody
AP32992HR-N

USD 550.00

2 Weeks

Mouse IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot. 
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections. 
In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions 
Histochemical and cytochemical Use: 1/100- 1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000- 1/5,000.
Reactivities Mouse
Conjugation HRP
Immunogen Purified homogenous IgM isolated from Mouse serum. Immunization with intact and split IgM.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgG (H+L chain) Sheep Polyclonal Antibody
R1256AP

USD 270.00

3 Days

Mouse IgG (H+L chain) sheep polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 �g of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Mouse IgG whole molecule.
Mouse IgG (H+L chain) Sheep Polyclonal Antibody
R1256B

USD 220.00

3 Days

Mouse IgG (H+L chain) sheep polyclonal antibody, Biotin

Applications Suiatble for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/1,000-1/2,000.
Conjugation Biotin
Immunogen Mouse IgG whole molecule.
Mouse IgG (H+L chain) Sheep Polyclonal Antibody
R1256F

USD 205.00

3 Days

Mouse IgG (H+L chain) sheep polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Mouse IgG whole molecule.
Mouse IgG (H+L chain) Sheep Polyclonal Antibody
R1256T

USD 205.00

3 Days

Mouse IgG (H+L chain) sheep polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgG whole molecule.
Mouse IgG (H+L chain) Sheep Polyclonal Antibody
R1256TR

USD 220.00

3 Days

Mouse IgG (H+L chain) sheep polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Mouse IgG whole molecule.
Chicken IgG / Chicken IgY (H+L chain) Rabbit Polyclonal Antibody
R1300B

USD 265.00

3 Days

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/500,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/5,000.
Conjugation Biotin
Immunogen Chicken IgG whole molecule.