Mouse IgG2b goat polyclonal antibody, HRP
| Applications | ELISA: 1/4000-1/8000. Western Blot. Immunohistochemistry on Frozen Sections Immunohistochemistry on Paraffin Sections. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Mouse IgG2b paraproteins |
Turkey IgG (H+L chain) goat polyclonal antibody, Biotin
| Applications | Immunofluorescence. ELISA. Western blotting. Dot- and Slot-Immunoblotting. Immunohistochemistry. |
| Conjugation | Biotin |
Turkey IgG (H+L chain) goat polyclonal antibody, FITC
| Applications | Immunofluorescence: < / = 1 µg/10e6 cells. ELISA. Western blotting. Dot- and Slot-Immunoblotting. Immunohistochemistry. |
| Conjugation | FITC |
Turkey IgG (H+L chain) goat polyclonal antibody, Purified
| Applications | Immunofluorescence. ELISA. Western blotting. Dot- and Slot-Immunoblotting. Immunohistochemistry. |
Turkey IgG (H+L chain) goat polyclonal antibody, PE
| Applications | Immunofluorescence. ELISA. Western blotting. Dot- and Slot-Immunoblotting. Immunohistochemistry. |
| Conjugation | PE |
Mouse IgG (H+L chain) goat polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/150,000. Western Blot: 1/3,000-1/15,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Mouse |
| Immunogen | Mouse IgG, whole molecule. |
Mouse IgG (Fc specific) goat polyclonal antibody, Ig Fraction
| Applications | ELISA: 1/20000-1/100000. Western Blot: 1/2000-1/10000. Immunohistochemistry: 1/1000-1/5,000. |
| Reactivities | Mouse |
| Immunogen | Mouse IgG F(c) fragment |
Human IgG (F(ab)2 specific) goat polyclonal antibody, FITC
| Applications | Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. Recommended Dilutions: 1/20-1/80. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Human |
| Conjugation | FITC |
| Immunogen | Purified Fab prepared from normal IgG isolated from pooled Human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgG (F(ab)2 specific) goat polyclonal antibody, TRITC
| Applications | Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. Recommended Dilutions: 1/10-1/40 This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Human |
| Conjugation | TRITC |
| Immunogen | Purified Fab prepared from normal IgG isolated from pooled Human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgA (Fc specific) sheep polyclonal antibody, TRITC
| Applications | Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgA. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. Recommended Dilutions: 1/10-1/40 This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Human |
| Conjugation | TRITC |
| Immunogen | Purified IgA prepared from pooled Human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgG (Fc specific) sheep polyclonal antibody, TRITC
| Applications | Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgG. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. Recommended Dilutions: 1/10-1/40 This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Human |
| Conjugation | TRITC |
| Immunogen | Purified IgG prepared from pooled Human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgE (Fc specific) goat polyclonal antibody, TRITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/20 - 1/60, depending on the method used. |
| Reactivities | Mouse |
| Conjugation | TRITC |
| Immunogen | Pools of purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgM (heavy chain) rabbit polyclonal antibody, Purified
| Applications | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10000-1/50000. Immunofluorescence: 1/1000-1/5000. Flow Cytometry: 1/500-1/2500. |
| Reactivities | Porcine |
| Immunogen | Swine IgM mu heavy chain |
Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, FITC
| Applications | Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/80. |
| Reactivities | Monkey |
| Conjugation | FITC |
| Immunogen | Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgG (H+L chain) goat polyclonal antibody, FITC
| Applications | To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/80. |
| Reactivities | Monkey |
| Conjugation | FITC |
| Immunogen | Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. |
| Reactivities | Monkey |
| Conjugation | FITC |
| Immunogen | Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgA (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/80, depending on the method used. |
| Reactivities | Canine |
| Conjugation | FITC |
| Immunogen | Pools of purified IgA isolated from dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgG (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgG isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/15 and 1/60, depending on the method used. |
| Reactivities | Canine |
| Conjugation | FITC |
| Immunogen | Pools of purified IgG isolated from dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgG (H+L chain) goat polyclonal antibody, FITC
| Applications | Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of dog origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/120. |
| Reactivities | Canine |
| Conjugation | FITC |
| Immunogen | Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgM (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. General Recommended Dilutions: are usually between 1/10 and 1/40, depending on the method used. |
| Reactivities | Canine |
| Conjugation | FITC |
| Immunogen | Purified IgM isolated from Dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: For histochemical and Cytochemical use are usually between 1/100 and 1/250 In ELISA and comparable non-precipitating antibody-binding assays between 1/1500 and 1/6000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Purified homogenous IgM isolated from Mouse serum. Immunization with intact (19S) and split IgM (7S). Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgM (Fc specific) goat polyclonal antibody, FITC
| Applications | In direct staining of cytoplasmic IgM in fixed mouse cells and tissue substrates; to identify circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/80. |
| Reactivities | Mouse |
| Conjugation | FITC |
| Immunogen | Purified homogenous IgM isolated from pooled mouse serum. Immunization with intact (19S) and split (7S) IgM. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgM (Fc specific) goat polyclonal antibody, TRITC
| Applications | Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/10 and 1/80. |
| Reactivities | Mouse |
| Conjugation | TRITC |
| Immunogen | Purified pools of homogenous IgM isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Aff - Purified
| Applications | ELISA: 1/10,000 dilutions produced a detectable signal, as measured using HRP-labeled Chicken anti-Goat IgG Antibody Cat.-No AP31796HR-N (1/5000 dilution). Western blot (1/5000). Immunocytochemistry. Immunohistochemistry (1/250). Immunoprecipitation (1/250). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and A30-106-10 (75 μl) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough. |
| Reactivities | Chicken |
| Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
Goat IgG (H+L chain) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA. Western Blot (1/1000). Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose. |
| Reactivities | Goat |
| Immunogen | Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant. Production. After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (whole protein). |
Mouse IgA (alpha chain specific) goat polyclonal antibody, HRP
| Applications | ELISA: 1/4,000-1/8,000. Immunoblotting. Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins. |
Mouse IgA (alpha chain specific) goat polyclonal antibody, Aff - Purified
| Applications | Immunoblotting. Flow Cytometry. Immunohistochemistry. Enzyme-Linked-Immunosorbent-Assay (ELISA). Fluorescent-Linked-Immunosorbent-Assay (FLISA). |
| Reactivities | Mouse |
| Immunogen | Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins. |
Mouse IgA (alpha chain specific) goat polyclonal antibody, PE
| Applications | FLISA. Flow Cytometry. Immunofluorescence: ≤ 0.1 µg/106 cells. Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | PE |
| Immunogen | Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins. |
Mouse IgA (alpha chain specific) goat polyclonal antibody, TRITC
| Applications | FLISA: 1/100-1/400. Flow Cytometry. Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | TRITC |
| Immunogen | Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins. |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, AP
| Applications | ELISA: 1/2,000-1/4,000. Immunoblotting. Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | AP |
| Immunogen | Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins. |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Aff - Purified
| Applications | Indirect Immunostaining (In conjunction with mouse monoclonal antibodies). |
| Reactivities | Mouse |
| Immunogen | Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins. |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, TRITC
| Applications | FLISA: 1/100–1/400. |
| Reactivities | Mouse |
| Conjugation | TRITC |
| Immunogen | Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins. |
Duck IgG (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Antibody titre: Precipitin titre not less than 1/128 when tested against normal Duck serum in agar block titration. |
| Reactivities | Duck |
| Immunogen | Purified normal Ig fractions isolated from pooled Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Duck IgG (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of duck origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/10-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000. |
| Reactivities | Duck |
| Conjugation | Biotin |
| Immunogen | Purified normal Ig fractions isolated from pooled Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea Pig IgG (Fc specific) sheep polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. |
| Reactivities | Guinea Pig |
| Conjugation | Biotin |
| Immunogen | Purified normal IgG isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea Pig IgG (Fc specific) sheep polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. |
| Reactivities | Guinea Pig |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea Pig IgM (Fc specific) sheep polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in guinea pig serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of guinea pig IgM from serum or plasma. Directions for Use: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/32 when tested against normal guinea pig serum in agar block titration. |
| Reactivities | Guinea Pig |
| Immunogen | Highly purified normal IgM isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (Fc specific) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used As unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5.000. Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. Can be used In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in sheep serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000. |
| Reactivities | Sheep |
| Conjugation | Biotin |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in sheep serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8.000. |
| Reactivities | Sheep |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000. |
| Reactivities | Sheep |
| Conjugation | Biotin |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Frozen Sections. Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Sheep |
| Conjugation | FITC |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in sheep serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000. |
| Reactivities | Sheep |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in sheep serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/4,000. |
| Reactivities | Sheep |
| Conjugation | Biotin |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in sheep serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. |
| Reactivities | Sheep |
| Conjugation | HRP |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000. |
| Reactivities | Sheep |
| Conjugation | Biotin |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000. |
| Reactivities | Sheep |
| Conjugation | HRP |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of swine origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in swine serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000. |
| Reactivities | Porcine |
| Conjugation | Biotin |
| Immunogen | Purified normal IgG isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of swine origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in swine serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. |
| Reactivities | Porcine |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
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