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GFAP chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse, Rat |
| Immunogen | Recombinant Human GFAP (expressed in bacteria). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks. |
Chicken Polyclonal mGFP Antibody
| Applications | WB |
| Immunogen | mGFP |
Chicken Polyclonal mKate Antibody
| Applications | WB |
| Immunogen | Purified mKate protein expressed in E. coli. |
Anti-MYC tag chicken polyclonal antibody
| Applications | WB |
| Immunogen | Synthetic peptide (EQKLISEEDL) conjugated to KLH |
Chicken Polyclonal mCherry Antibody
| Applications | WB |
| Immunogen | mCherry |
Anti-DDK (FLAG) chicken polyclonal antibody
| Applications | WB |
| Immunogen | Synthetic peptide (DYKDDDDK) conjugated to KLH |
Anti-Tdtomato chicken polyclonal antibody
| Applications | WB |
| Immunogen | Purified Tdtomato protein expressed in E. coli. |
Chicken polyclonal eGFP antibody
| Applications | IHC, WB |
| Immunogen | Green fluorescent protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria |
Chicken Polyclonal mPlum Antibody
| Applications | WB |
| Immunogen | Purified mPlum protein expressed in E. coli. |
Anti-HA tag chicken polyclonal antibody
| Applications | WB |
| Immunogen | Synthetic peptide (YPYDVPDYA) conjugated to KLH |
Chicken polyclonal anti-TUBA1A(alpha Tubulin) antibody, Loading control
| Applications | WB |
| Reactivities | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Immunogen | Tubulin antibody was raised against a 16 amino acid peptide near the amino terminus of human Tubulin. |
GFP chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Immunogen | Purified recombinant Green Fluorescent Protein (GFP) emulsified in Freund's adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Purified antibody preparations were then mixed with Glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with Thimerosal, and then filter-sterilized. |
lacZ chicken polyclonal antibody, Purified
| Applications | IF, IHC, WB |
| Immunogen | Purified Beta-Galactosidase (LacZ from E.coli) protein emulsified in Freund's adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using Beta-Galactosidase conjugated to an Agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 mg/ml) and purified IgY (10 mg/ml), and is filter-sterilized. |
Chicken Polyclonal Albumin Antibody
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Immunogen | Albumin antibody was raised against a 13 amino acid synthetic peptide near the center of human Albumin. The immunogen is located within amino acids 340 - 390 of Albumin. |
Chicken Polyclonal Anti-GAPDH Antibody
| Applications | IF, WB |
| Reactivities | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Immunogen | GAPDH antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human GAPDH. The immunogen is located within amino acids 230 - 280 of GAPDH. |
Chicken Anti-Microtubule Associated Protein 2 (MAP2) Antibody
| Applications | WB |
| Reactivities | Bovine, Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Immunogen | Bovine MAP2 isolated from brain by the GTP microtubule cycling method |
Bone Sialoprotein (IBSP) (native) chicken polyclonal antibody, Purified
| Applications | ELISA, FC, IF, IHC, R, WB |
| Reactivities | Human |
| Immunogen | Human Bone Sialoprotein (bone extract) |
Bone Sialoprotein (IBSP) chicken polyclonal antibody, Purified
| Applications | ELISA, FC, IF, IHC, R, WB |
| Reactivities | Human |
| Immunogen | Human Bone Sialoprotein (bone extract) |
DYKDDDDK Epitope Tag chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IF, IHC, IP, WB |
| Immunogen | Synthetic peptide containing the DYKDDDDK epitope conjugated to Keyhole Limpet Hemocyanin (KLH). Production: The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were then affinity-purified over a peptide column and dialyzed against Phosphate-Buffered isotonic Saline. Sodium azide was added, the protein concentration was adjusted and the antibodies were filter-sterilized (0.2 μm). |
c-Myc (MYC) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IF, IHC, IP, WB |
| Reactivities | Human |
| Immunogen | Synthetic peptide containing the Human c-myc epitope (i.e., EQKLISEEDL) conjugated to KLH. The first injection included complete Freund's adjuvant; subsequent injections included incomplete Freund's adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. |
Complement C3 (C3) chicken polyclonal antibody, HRP
| Applications | ELISA, FC, WB |
| Reactivities | Human |
| Conjugation | HRP |
| Immunogen | Purified Human Complement Component 3a (C3a) peptide. |
Goat IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | ELISA: a 1/75,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot (1/5000). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in Agarose gels and ELISA analysis. |
| Reactivities | Goat |
| Conjugation | HRP |
| Immunogen | Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant. Production: After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase. |
Human IgG (H+L chain) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA. Western Blot (1/1000). Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose. |
| Reactivities | Human |
| Immunogen | Purified, non-denatured Human IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains). |
Rabbit IgG (H+L chain) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA. Western Blot (1/1000). Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose. |
| Reactivities | Rabbit |
| Immunogen | Purified, non-denatured Rabbit IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains). |
GAD67 (GAD1) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse, Rat |
| Immunogen | Synthetic peptide KLH conjugated corresponding to a region near the C-terminus of this gene product, and was 100% conserved between the Human (Q99259), Mouse (P48318) and Rat (NP_058703) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column. |
MAP2 chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | Two synthetic peptides KLH conjugated corresponding to different regions of the MAP-2 gene product, but are shared between the Human (NP_002365) and Mouse (P20357) sequences. Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/ml. Finally, equal volumes of both of these affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Myelin Basic Protein (MBP) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | Synthetic peptide KLH conjugated corresponding to a region of the MBP gene product shared between the Human (NP_002376) and Mouse (NP_034907) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. |
Nes chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Mouse, Rat |
| Immunogen | Three synthetic peptides KLH conjugated corresponding to different regions of the Mouse Nestin gene product (NP_057910), but are shared with the Rat (NM_012987) protein sequence. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
GDNF Receptor alpha 1 (GFRA1) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IF, WB |
| Reactivities | Human |
| Immunogen | Recombinant His-tagged human GFRa-1 protein produced using CHO-based. For production of hGFRa-1, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant. |
Chicken Polyclonal Anti-Beta-actin Antibody
| Applications | WB |
| Reactivities | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Immunogen | Beta-actin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human beta-actin. |
GFP (Ads. to Hu, Ms, Rt Serum Proteins) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IF, WB |
| Reactivities | A. victoria |
| Immunogen | Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria |
HA Epitope Tag (YPYDVPDYA) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IF, IHC, WB |
| Immunogen | Synthetic peptide containing the Influenza Hemaglutinin epitope (YPYDVPDYA) conjugated to Keyhole Limpet Hemocyanin (KLH). |
c-Myc (MYC) chicken polyclonal antibody, FITC
| Applications | ELISA, IF, IHC, WB |
| Reactivities | Human |
| Conjugation | FITC |
| Immunogen | Synthetic peptide containing the Human c-myc epitope (i.e., EQKLISEEDL) conjugated to KLH. After repeated injections, immune eggs were collected, from which the IgY fractions were prepared. |
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