Ku80 (XRCC5) Rabbit Polyclonal Antibody

CAT#: TA355656

XRCC5 Antibody - C-terminal region

Product Images

WB Suggested Anti-XRCC5 Antibody Titration: 1.25ug/ml ELISA Titer: 1:12500 Positive Control: Jurkat cell lysateXRCC5 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells

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  Lanes: 1. 30 ug MiaPaca-2 cell lysate 2. 30 ug Panc-1 cell lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Goat anti-Rabbit HRP Secondary Antibody Dilution: 1:4000 Gene Name: XRCC5 Submitted by: Dr. Crissy Dudgeon, Ph.D., CINJ

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  Human Pancreas

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  Sample Type: Human lung adenocarcinoma cell line A549 Primary Antibody Dilution: 1:100 Secondary Antibody: Goat anti-rabbit AlexaFluor 488 Secondary Antibody Dilution: 1:400 Color/Signal Descriptions: XRCC5: Green DAPI:Blue Gene Name: XRCC5 Submitted by: Dr. Crissy Dudgeon, Ph.D., CINJ

Specifications

Product Data

• Applications: IHC, WB
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human XRCC5
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
  Homology: Cow: 92%; Dog: 92%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 85%; Rabbit: 93%; Rat: 100%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: lot specific
• Purification: Protein A purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Predicted Protein Size: 83kDa
• Gene Name: X-ray repair complementing defective repair in Chinese hamster cells 5
• Database Link:
  NP_066964
  Entrez Gene 7520 Human
  UniProt ID Q4VBQ5
• Background: XRCC5 encodes the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
• Synonyms: CTC85; CTCBF; FLJ39089; G22P2; KARP-1; KARP1; Ku80; Ku86; KUB2; NFIV; OTTHUMP00000164061; OTTHUMP00000206791; TLAA

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Non-homologous end-joining