VP2 (NC_012798) Virus Tagged ORF Clone

CAT#: VC100645

Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human cosavirus E], codon optimized for human cell expression, YP_002956078

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100645 represents NCBI reference of YP_002956078 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCCCTCTGCCGAGAACTCTACCAACATGGGCGACCGCATTCTGACCCTCCGGGAAGGAAATACAG
  CGATTAGCTCTCAGGCTACAGAGGGTATTTTGCACGGGTACAATAAAGAAACGGACACTAGCCAGCCGAC
  CTCCTGTGGAGATAGGGCCTCTAAAGCCACCCCCGCCACCCAACGATCCTTTATCATTCCCTTGAAGGAC
  TGGACCAGTTCCCAGAATAGCTACTCTGCCCTCGTGTACAGAACGAGCCACATGTCTAAGGATGCCGTCG
  GGAACTTGTTCTCTAAAAACATGAACACCCACGCTTATACCAAGACTGGGTGGACAGCAACACTTCAAAT
  CAATACTTCTCCCTTTCATAGCGGGTTGATCGGCCTTTTTATGGTACCCGAATTCGTGAGAAAAATTTCT
  GATGACTTGGAATGGATTGATCTCGTCGCAGCAGGTTTTAACACGACGCTCTTTAAGGACTCAAATACAT
  ATAACCAGGACAGCGCTCTGGTCGGAAAAATCGATGACAAAAACACCTTTGACCTCGCGTCCACCACGCC
  CGAACAGTTCTTTCTGTATCCTCACCAACTTATTAATCCAAAAGAGACTACCATGGCTACTGTGTCAGTG
  CCTTACGTAAATGTCGCACCATCTTCCGATCCTTCTGTCCACAACATATGGACAGTCGTCGTCATGGTGC
  TGTCCCCCCTTCAGATTGGGAGCGGGGCTTCCCCCAACGTGCAGATGACACTCACTCTGACCCCCACAGG
  AAGCGTGTTTAATGGACTTCGGCACCCTAAACCAGCCGCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100645 representing YP_002956078
  Red=Cloning sites Green=Tags
  
  MTPSAENSTNMGDRILTLREGNTAISSQATEGILHGYNKETDTSQPTSCGDRASKATPATQRSFIIPLKD
  WTSSQNSYSALVYRTSHMSKDAVGNLFSKNMNTHAYTKTGWTATLQINTSPFHSGLIGLFMVPEFVRKIS
  DDLEWIDLVAAGFNTTLFKDSNTYNQDSALVGKIDDKNTFDLASTTPEQFFLYPHQLINPKETTMATVSV
  PYVNVAPSSDPSVHNIWTVVVMVLSPLQIGSGASPNVQMTLTLTPTGSVFNGLRHPKPAAQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012798
• ORF Size: 813 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012798.1, YP_002956078
• RefSeq ORF: 813
• MW: 29.5 kDa