2B (NC_012798) Virus Tagged ORF Clone

CAT#: VC100649

Myc-DDK-tagged ORF clone of viral ORF for 2B [Human cosavirus E], codon optimized for human cell expression, YP_002956082

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100649 represents NCBI reference of YP_002956082 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAACTTCTCAAAGTTTTGTGTCCAGGGGCCGACCAGCGACTTCATTCGCCTGGTGCGCGACCCAG
  AAACACTGGATAATGTCAATAGACTGCTCTCCACACTGAACAACCTGATGGCCAAATGGAACAGTTTCAA
  GCATATGTGTCTGGATTCCTATTTTCTGAGAGACATTCTCTGCCTGCTGACGAAGCTCACAGCCCTTGGC
  TTTCTGGTCCACAACCAGGGACCCTCAGCCTATCTGGCCGCCGCAGCCATTTTGATTTCAGATGGGATTA
  GCTTTCTGGACTGGTACGATAAGATCAAAAGGTTTATGAGCAGGAGGCTGCGGATCTCACCCCCCCCATT
  TTGCTTGGCGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100649 representing YP_002956082
  Red=Cloning sites Green=Tags
  
  MPNFSKFCVQGPTSDFIRLVRDPETLDNVNRLLSTLNNLMAKWNSFKHMCLDSYFLRDILCLLTKLTALG
  FLVHNQGPSAYLAAAAILISDGISFLDWYDKIKRFMSRRLRISPPPFCLAQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012798
• ORF Size: 363 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012798.1, YP_002956082
• RefSeq ORF: 363
• MW: 13.9 kDa