3C (NC_012798) Virus Tagged ORF Clone

CAT#: VC100653

Myc-DDK-tagged ORF clone of viral ORF for 3C [Human cosavirus E], codon optimized for human cell expression, YP_002956086

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100653 represents NCBI reference of YP_002956086 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACCATTGCTGGATCTGGAGAAAAAGATCATGAACAACGTGCAGCCCTTTGTCATGCGGTTCGGAA
  ACAGGGAGTACGTGCAGTCTTGCCTGTTCGTAGGAAAAAGGGTCTTTCTTGTCAACAAACATGCTATTGA
  AACAGTCGAGGAAAGATTTAGTGTTGCCGGCACGGAATACAATATCGATGATGTCGAAATCGCGATTCTG
  GACACCGAATACGGTCTGACTGACGTAGCAGCCGTCAAGATCTCAAACGGCCCCGAGTGGAAAAACCTCT
  CTAAGTTGTTCCTGAATCTCGAAACAGAACTCTTGCCTGGCACTCGCATCACAGTTCTGTCAAACGACAA
  CATTAACATGCTGCGGGAAGGTAGCTTCCTGAGGTACGAGGAGGAAATCCCAACTAACATTGGCCCCATT
  CCCTTTGTGATGCTGTACAAAGCAAGTAGTTACTTTGGCATGTGCGGCTCAGCAGTTGTAACAAGATTCG
  GCGATTCATCAGGGATTCTGGGAATCCATTGTGCCGGCGGTGGCGGAGTAAGTGTGGCGAGCCGCGTGAC
  CCGGAAGATGGTCGAAACTGTACTGGAGTATTTCTATCCACCACAGGTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100653 representing YP_002956086
  Red=Cloning sites Green=Tags
  
  MSPLLDLEKKIMNNVQPFVMRFGNREYVQSCLFVGKRVFLVNKHAIETVEERFSVAGTEYNIDDVEIAIL
  DTEYGLTDVAAVKISNGPEWKNLSKLFLNLETELLPGTRITVLSNDNINMLREGSFLRYEEEIPTNIGPI
  PFVMLYKASSYFGMCGSAVVTRFGDSSGILGIHCAGGGGVSVASRVTRKMVETVLEYFYPPQVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012798
• ORF Size: 612 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012798.1, YP_002956086
• RefSeq ORF: 612
• MW: 22.7 kDa