VP1 (NC_012798) Virus Tagged ORF Clone

CAT#: VC100647

Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human cosavirus E], codon optimized for human cell expression, YP_002956080

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100647 represents NCBI reference of YP_002956080 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACACGACAACGCAGAGAAGGGTACCAGTTCTAACCACGATGCCTCCCTGACCGGAGGCCATAGTG
  TAGGAATTAAGGTAGCACACTCCGACACAAGCTTTTTCTTTGACCGATATAGATATATTGGAACCGTGGA
  GTCAACAAAAAACACCCCTGCCACCGTTGCTACGCTGACTGACACTAACGGAATTATCAAGAGCTTCAAC
  AATCTTGCCAATGCCGATAATGGATACAAGAAAGTTTTGATGCTGGCGCCTAATCCCTCATTTTGTGGGG
  CCGCATACACAGTCTTCGCTATGTTCGACCAAACCGGCACCACCGGCGCTAAGAAGCGCTTGCTGTTTGG
  ACAGGGGGACTACCACCTGTATAGGTCTTGCCCTTTCACCTACTACACCGCCGATCTCGAAGTTACTGTC
  GTGCCAGACGCAGGAGTGGCGGGACCATGGAGGGTTACTTGGCTTCCCGTTGGCGCCCCGCTCGATGAGA
  TCGAAATGATTACCAGCGAGACATCAAATCCCCAGTCTTGGAGTTTCTTCACTGAGGATAGGAAGGACTC
  TTCCTCTCTCTACAGCCTCAACCCCACCTTCTGGTCTAACGATCAAAATAAGGTGTCTTTCGATATTCCT
  ATGACCACCCCTCTGACTCTGTTGCCCTTGTACTTCGACGGTTACCCCGACTATAGCAATACAAAGGGCC
  TGTATGGTGTAGCCCCAGCTAACGGCTTCGGCAACATCGTGGTAAAGTCCAATACCCTGGCTGACAAGTT
  TATGGTTTACATCAGATACAAAAACTTTAGGGGGTACGTGCCAAGGCCACTGCCCCGGCTGGTTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100647 representing YP_002956080
  Red=Cloning sites Green=Tags
  
  MPHDNAEKGTSSNHDASLTGGHSVGIKVAHSDTSFFFDRYRYIGTVESTKNTPATVATLTDTNGIIKSFN
  NLANADNGYKKVLMLAPNPSFCGAAYTVFAMFDQTGTTGAKKRLLFGQGDYHLYRSCPFTYYTADLEVTV
  VPDAGVAGPWRVTWLPVGAPLDEIEMITSETSNPQSWSFFTEDRKDSSSLYSLNPTFWSNDQNKVSFDIP
  MTTPLTLLPLYFDGYPDYSNTKGLYGVAPANGFGNIVVKSNTLADKFMVYIRYKNFRGYVPRPLPRLVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012798
• ORF Size: 837 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012798.1, YP_002956080
• RefSeq ORF: 837
• MW: 30.9 kDa