2C (NC_012798) Virus Tagged ORF Clone

CAT#: VC100650

Myc-DDK-tagged ORF clone of viral ORF for 2C [Human cosavirus E], codon optimized for human cell expression, YP_002956083

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100650 represents NCBI reference of YP_002956083 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCTTGATCTGCGAGATGTGGTTACATTCTTTAACGCTGCTAAGGGCGCCCAGTGGATGGTGGACT
  CTATCCGAAGTTTGATCCGCTGGATTAAGCAGTGGCTGGAGCTGGAGGAAGAAAACGAAGCAGTGCAATT
  TGAGAAAATGCTGGTAGACTCCCCAAAACATTGCAAGGCTATAAACGATTACAATGTAGGGAAGACCTTC
  GTTCGCCCTGAAAATTCATTCGATTTCATGGATAAACTGGTAGACTCCGCAACTAAGCTTGGCCGGGTCA
  ACATCGCGAGTTACTTTCGGAGCTTCACCTCCGTTGATACTGATGCTCCCCGGATGGAGCCCGTTGTGCT
  GGTACTGCGGGGGAAGCCCGGCGCCGGCAAATCCGCAGCTGCCACCGTCATCGCTGCTGCCGTATCCAAG
  ACACTCACCGGCACACAGAGCGTCTATACTCTGAGTCCGGATACCGAGCACATGGACGGTTACCACGGCC
  AGTTCTCAATGATCATGGACGACCTTGGCCAGAACCCTGATGGGGAAGATTTTAGGACCTTTTGTCAAAT
  GATTTCTTGCGCCCAGTACAGGCCCTCTATGGCCGATCTCCGGGACAAAGGCATTCTCTTCAAGAGCCAG
  TTTATAATAGCAACTACCAATCTTCCCGAGTTTCGACCTCTTACGATAAGCGACAGAGGGGCAGTCGAGA
  GGAGGATAACTTTTGACATTGGCGTGACTCCTGGAAGCGCCGCTACGAGGAATGGGAAACTGGATCTGGC
  TATCGCCCTCAAACCCGATGGACCTGGCGAGTTCCCTTATAACTGCGACTGCCAGCTGCTCCATACCACC
  GGCCTCGCCCTGCAGAACCTGCGATCAGGAAAGACTATGAACTTGAAAGAACTCGTGGACATGATTGTGA
  AGAAGATTAAATCTAAACGGACCACCAGCGGTATGCTGGAAGGACTTGTAATGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100650 representing YP_002956083
  Red=Cloning sites Green=Tags
  
  MGLDLRDVVTFFNAAKGAQWMVDSIRSLIRWIKQWLELEEENEAVQFEKMLVDSPKHCKAINDYNVGKTF
  VRPENSFDFMDKLVDSATKLGRVNIASYFRSFTSVDTDAPRMEPVVLVLRGKPGAGKSAAATVIAAAVSK
  TLTGTQSVYTLSPDTEHMDGYHGQFSMIMDDLGQNPDGEDFRTFCQMISCAQYRPSMADLRDKGILFKSQ
  FIIATTNLPEFRPLTISDRGAVERRITFDIGVTPGSAATRNGKLDLAIALKPDGPGEFPYNCDCQLLHTT
  GLALQNLRSGKTMNLKELVDMIVKKIKSKRTTSGMLEGLVMQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012798
• ORF Size: 966 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012798.1, YP_002956083
• RefSeq ORF: 966
• MW: 35.7 kDa