VP2 (NC_013114) Virus Tagged ORF Clone

CAT#: VC100705

Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human enterovirus 98], codon optimized for human cell expression, YP_003104776

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100705 represents NCBI reference of YP_003104776 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCCCTCTGCAGAGGAATGCGGGTATTCTGATCGCGTACGGAGCATCACGCTCGGTAACAGCACCA
  TCACCACTCAGGAGTGTGCTAACGTGGTGGTGGCGTATGGAGAATGGCCAAACTACCTCCGGGATGATGA
  AGCCACTGCTGAGGATCAGCCAACCCAGCCTGACGTGGCCACCTGCAGGTTCTACACCCTTGATAGTGTG
  GACTGGCAGAAAAACAGTGCGGGATGGTGGTGGAAGTTCCCTAATGCGTTGAAAGACATGGGGCTTTTTG
  GCCAGAACATGCTGTACCATTACCTGGGGAGAACAGGCTACACCGTTCACGTGCAATGCAATGCTTCTAA
  GTTTCACCAGGGATGCCTGCTGGTTGTCTGCGTTCCGGAGGCAGAAATGGGATGTGCAACTGTCGACAGG
  ACTGTTACTGAGGAATATTTGTCAAAGGGAGAGCAACCTTGCAACTTCACAACGACCAATGCCACAGGGT
  CCAATGAGGTGCAAACAGCCGTACATAACGCTGGCATGGCCGTCGCAGTGGGGAACTTGACAATCTTTCC
  GCACCAGTGGATAAACCTGAGAACCAATAACTCCGCTACTATCGTTATTCCATACATCAATTCTGTGCCT
  ATGGATAATATGTACAGGCATCAAATTTTCACATTGATGATCATACCCTTTGTCCCACTGGACATGAGCA
  CAGGGGCTTCCAGTGTGGTCCCTATCACCGTGACAGTGGCCCCCATGTCAGCCGAATACTACGGTCTGCG
  GCTCGCTTCTAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100705 representing YP_003104776
  Red=Cloning sites Green=Tags
  
  MSPSAEECGYSDRVRSITLGNSTITTQECANVVVAYGEWPNYLRDDEATAEDQPTQPDVATCRFYTLDSV
  DWQKNSAGWWWKFPNALKDMGLFGQNMLYHYLGRTGYTVHVQCNASKFHQGCLLVVCVPEAEMGCATVDR
  TVTEEYLSKGEQPCNFTTTNATGSNEVQTAVHNAGMAVAVGNLTIFPHQWINLRTNNSATIVIPYINSVP
  MDNMYRHQIFTLMIIPFVPLDMSTGASSVVPITVTVAPMSAEYYGLRLASKQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013114
• ORF Size: 786 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013114.1, YP_003104776
• RefSeq ORF: 786
• MW: 29.0 kDa