VP3 (NC_013114) Virus Tagged ORF Clone

CAT#: VC100706

Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human enterovirus 98], codon optimized for human cell expression, YP_003104777

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100706 represents NCBI reference of YP_003104777 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACTGCCAGTTCTCTGTACACCAGGCAGCAATCAGTTTCTTACGTCTGATGACTTTCAGTCCCCAT
  CTGCCATGCCCCAGTTCGATGCAACCCCCCAAATGCACATCCCAGGACAGGTGCGAAACCTCATGGAAAT
  CGCAGAGGTGGACTCCGTAGTGCCTGTGAATAACCTCTCTCGCTATGTCTCCACAATGGAAGCCTACCAG
  ATTCCCGTGTCTACAGGATCCCACACAACGACTAAGGCTATCTTTTCCTTCCAGCTCGACCCTGGAAATA
  GCAGTGTCATGTCACGCACACTTTTGGGTGAAATCCTTAACTACTACGCTCACTGGTCCGGATCCCTGAA
  ATTCACCTTCCTGTTCTGCGGCACTGCCATGGCTACAGGAAAGATCCTTCTTGCCTACTCACCGCCGGGC
  GCATCTGTACCAACCGACCGAAAACAAGCCATGTTGGGGACACATGTTATTTGGGACGTCGGACTCCAAT
  CATCTTGTGTACTGTGCGTACCATGGATCTCACAGACCCACTACCGATTTGTGCACGACGATGTGTACAC
  TTCCGCAGGATACATAACCGCCTGGTACCAGACAACAATCGTTGTGCCACCAAAGACCCCAACCGACTGC
  AAGCTGATGTGCTTCGTCAGTGCTTGTAATGATTTCTCTGTCAGGATGCTGAGAGACACACCTCACATCG
  AGCAGGACTCAGAACTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100706 representing YP_003104777
  Red=Cloning sites Green=Tags
  
  MGLPVLCTPGSNQFLTSDDFQSPSAMPQFDATPQMHIPGQVRNLMEIAEVDSVVPVNNLSRYVSTMEAYQ
  IPVSTGSHTTTKAIFSFQLDPGNSSVMSRTLLGEILNYYAHWSGSLKFTFLFCGTAMATGKILLAYSPPG
  ASVPTDRKQAMLGTHVIWDVGLQSSCVLCVPWISQTHYRFVHDDVYTSAGYITAWYQTTIVVPPKTPTDC
  KLMCFVSACNDFSVRMLRDTPHIEQDSELQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013114
• ORF Size: 720 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013114.1, YP_003104777
• RefSeq ORF: 720
• MW: 26.5 kDa