VP1 (NC_013114) Virus Tagged ORF Clone

CAT#: VC100707

Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human enterovirus 98], codon optimized for human cell expression, YP_003104778

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100707 represents NCBI reference of YP_003104778 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGACAAGATAGAAAACACTCCTACCGTTGCCGATACACAGCCTAGCGGAGCGCGAAACACGGAAC
  AGGTGCCTGCACTTACTGCCGTGGAAACAGGTCATACATCTCAAGTGGAACCGAGTGATACCGTTCAAAC
  CCGGCATGTTTGTAACTATCACACACGGAGCGAGAGCAGTGTGGAAAATTTCTTTGGCAGGGCTGCTTGT
  GTCAAAGTGCGGACGTACAACATTGGCCCAAACATTAAGGAAGAGGATCGCTACTCAGACTGGACCCTGT
  CAGTGAGGGACATGGTGCAAATTCGCCGGAAATTTGAAATGTTTACATATATGCGGTTCGACATAGAGAT
  TACCATTGTGGTGACATCCTATCAGCAGCCCGGGACAATTACCACCCAAGACATGCCAGTACTCACGCAC
  CAGTTTATGTATGTCCCTCCAGGTGGCCCAATACCAACCAGTGCAAGCAGCTTTGCGTGGCAGACCAGTA
  CTAATCCATCCGTCTTTTGGACCGAGGGAAACGCCCCAGCCCGATTGTCTATTCCTTTTGTCTCCGTGGG
  CAACGCTTACTCCTCATTTTACGATGGTTGGTCTCACTTTAATCAGTCCGGAGTGTATGGTTTCAACACC
  CTGAATAACATGGGCAGGCTGTTTATGAGGCACGTTAATAAGCTCTGTCCTAATCAGATTGTTAGTACAG
  CAAGGGTCTACCTGAAACCGAAGCATGTGAAAGCTTGGGTCCCCCGACCTCCCCGACTCTGTCCGTACAT
  CAACCGGGCCAACGTAAATTTCCATCCAACCGCTGTGGCTGATGTCCGCGCCGACATTAACGCTGTTCCC
  TCTCTGGAACATGAGCCTCTGCACACTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100707 representing YP_003104778
  Red=Cloning sites Green=Tags
  
  MGDKIENTPTVADTQPSGARNTEQVPALTAVETGHTSQVEPSDTVQTRHVCNYHTRSESSVENFFGRAAC
  VKVRTYNIGPNIKEEDRYSDWTLSVRDMVQIRRKFEMFTYMRFDIEITIVVTSYQQPGTITTQDMPVLTH
  QFMYVPPGGPIPTSASSFAWQTSTNPSVFWTEGNAPARLSIPFVSVGNAYSSFYDGWSHFNQSGVYGFNT
  LNNMGRLFMRHVNKLCPNQIVSTARVYLKPKHVKAWVPRPPRLCPYINRANVNFHPTAVADVRADINAVP
  SLEHEPLHTH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013114
• ORF Size: 870 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013114.1, YP_003104778
• RefSeq ORF: 870
• MW: 32.7 kDa