3C (NC_013114) Virus Tagged ORF Clone

CAT#: VC100713

Myc-DDK-tagged ORF clone of viral ORF for 3C [Human enterovirus 98], codon optimized for human cell expression, YP_003104784

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100713 represents NCBI reference of YP_003104784 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCTGCTTTCGAGTTCGCCGTAGCCATGATGAAACGCAACGCAAGTACTGTGAAGACGGAGTACG
  GTGAATTCACCATGCTTGGGATTTACGATAGGTGGGCAGTACTCCCAAGGCATGCCAAACCAGGACCCAC
  GATCCTGATGAATGATCAGGAGGTCGGTGTCGTGGATGCAAAGGAGCTCGTGGATAAGGACGGGACAAAC
  CTGGAACTTACACTGCTGAAACTGAACAGGAATGAAAAGTTTAGGGACATCAGGGGATTCTTGGCCAGAG
  AGGAAGCCGAAGTGAATGAGGCCGTACTCGCAATCAATACTTCCAAGTTCCCCAACATGTATATTCCTGT
  GGGGCAAGTCGCCGATTACGGTTTCCTGAATCTGGGCGGGACCCCAACCAAGCGGATGCTGATGTACAAC
  TTTCCAACACGCGCCGGTCAGTGTGGTGGCGTGCTCATGAGTACAGGAAAGGTCCTGGGCATCCACGTTG
  GCGGCAATGGACATCAGGGATTCTCCGCAGCTTTGCTTAGGCATTACTTCAACGATGAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100713 representing YP_003104784
  Red=Cloning sites Green=Tags
  
  MGPAFEFAVAMMKRNASTVKTEYGEFTMLGIYDRWAVLPRHAKPGPTILMNDQEVGVVDAKELVDKDGTN
  LELTLLKLNRNEKFRDIRGFLAREEAEVNEAVLAINTSKFPNMYIPVGQVADYGFLNLGGTPTKRMLMYN
  FPTRAGQCGGVLMSTGKVLGIHVGGNGHQGFSAALLRHYFNDEQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013114
• ORF Size: 552 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013114.1, YP_003104784
• RefSeq ORF: 552
• MW: 20.4 kDa